
Calyculin A is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phosphoserine/threonine, cat.# PP2551, as compared to untreated, control cell lysates.
C2C12 is a mouse muscle myoblast cell line that is useful for studies of myoblast and osteoblast differentiation. Confluent cultures of C2C12 cells were serum starved for 2 hours. Cells were then either left untreated (Cat.# CL9511) or treated with Calyculin A (100 nM) for 30 minutes at 37°C (cat.# CL9521). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
This kit contains:
KIT SUMMARY
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
E.coliExpressionSystems.pdf(136.05k)
基因重组只是控制同一性状表达不同而已,但是它不会改变性状的基本情况。如:控制眼睛颜色的基因,亲本的基因重组之后可能亲本为黑色,子代便为蓝色。但绝对不会控制眼睛颜色的基因在重组之后就变成控制耳朵的基因。
而没答案
帮帮我
给我提供答案
考研细胞的题答案
你可以试着读一下看看。
或者可以过柱纯化,过柱纯化要加一半的佐剂,所以纯化后浓度要高于500ug/ml为宜,否则要增加免疫的次数。
生物制品细胞制备过程中不得使用青霉素或β-内酰胺(β-Lactam)类抗生素,明确表明不准使用青霉素。那么进行的重组蛋白类表达的微生物发酵,可以用青霉素吗?
2、2015版药典三部,各论中指出,”将检定合格的工作种子批菌种接种于适宜培养基(可含适量抗生素)中培养“,”发酵用培养基(采用适宜的不含抗生素的培养基)“。
这里说明种子培养基中可以使用抗生素,而且也没有限定究竟是哪种抗生素。
3、关于发酵中使用的抗生素,不知大家一般用哪家的,一些常用的,氨苄西林钠,硫酸卡那霉素,氯霉素等,都是用的什么级别,那个厂家的呢?我现在生工的,阿拉丁的,生兴生物的都用过。主要还是用的生工生物的,但是生工生物的抗生素都是进口分装的,不仅仅抗生素,国内很多进口分装的试剂,都无法提供相应的产品质量检测证书。而且因为是分装的,基本也就属于只有销售,所以,以后真的生产了,原材料来源的稳定性也无法保证。请问这个问题,大家是怎么解决的,一般用的哪家的呢。

