Type
Native
Description
Nativeproteinisolatedfrompooledcanineserum,347 AA.MW:38,129 kDa(calculatedwithoutglycosylation).ProteinidentityconfirmedbyLC-MS/MS(NCBIno.gi|73987375).
AminoAcidSequence
MSPWSPERSQSPGGLSSHLSKTLLLLLFSVASAQGVTPDRDTCLVFPSSNGSSVSCHPPAKLPHHFPSDTVHLVVEFFNLTQLRPDTLQGVSNLQELHLSTNQLESLSPKLLLPVPLLKVLDLTRNALTQLPPGLFRVSAALHTLVLKENRLEVLEPSWLCGLKALGHLDLSGNHLQTLPPGLLANVTALRILDLSNNQLKTLPLDLLRGPLQLERLHLEGNRLQVLEEGLLAPQPGLRYLFLSDNKLAAVAAGAFRGLQQLDMLDLSNNSLTSVPKGLWTSLGQPTRDMEDGFDISGNPWICDHNLDDLYRWLVANKDKMFSRNDTRCARPEALKGQTLLEAAESH
Source
Caninepooledserum
Purity
>90%
SDS-PAGEGel
SDS-PAGEanalysisofCanineLeucine-richalpha-2-glycoprotein-1 nativeprotein,12%gelstainedwithCoomassieBrillantBlueR250
- M.W.Marker –14,21,31,45,66,97 kDa
- reducedandboiledsample,2.5μg/lane
- non-reducedandnon-boiledsample,2.5μg/lane
BIOLOGicalActivity
ProteinspecificallybindtohumanCytochromec1,hemeprotein –tested.
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mlin20mMTRIS,50mMNaCl,pH 8.0
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies,Immunologicalmethods
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
