Type
Recombinant
Description
Midkine(MK)anditsfunctionally-relatedproteinpleiotrophinareheparin-bindingneurotrophicfactorsthatsignalthroughthesamereceptor,knownasanaplasticlymphomakinase(ALK).MKplaysanimportantregulatoryroleinepithelial-mesenchymalinteractionsduringfetaldevelopmentandinpostnatallungdevelopment.MKchemoattractsembryonicneurons,neutrophilsandmacrophages,andexertsangiogenic,growthandsurvivalactivitiesduringtumorgenesis.RecombinantMurineMidkineisa13.3 kDaproteincontaining120 aminoacidresiduesincludingfiveintra-moleculardisulfide bonds.
AminoAcidSequence
VAKKKEKVKKGSECSEWTWGPCTPSSKDCGMGFREGTCGAQTQRVHCKVPCNWKKEFGADCKYKFESWGACDGSTGTKARQGTLKKARYNAQCQETIRVTKPCTSKTKSKTKAKKGKGKD
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytochemoattracthumanneutrophilsusingaconcentrationrangeof10–100 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C