Type
Recombinant
Description
MIP-3αisaCCchemokine,expressedintheliver,lymphnodes,appendix,PBLandlungsthatcansignalthroughtheCCR6receptor.MIP-3αischemotactictowardslymphocytesanddendriticcells.Additionally,itpromotestheadhesionofmemoryCD4+Tcells,andinhibitscolonyformationofbonemarrowmyeloidimmatureProgenitors.RecombinantMurineMIP-3αisa7.9 kDaproteincontaining70 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.
AminoAcidSequence
ASNYDCCLSYIQTPLPSRAIVGFTRQMADEACDINAIIFHTKKRKSVCADPKQNWVKRAVNLLSLRVKKM
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytochemoattractmurineCCR6transfectedHEK/293cellsusingaconcentrationrangeof0.1–10.0 ng/ml
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C