Type
Recombinant
Description
Total201 AA.MW:23.6 kDa(calculated).UniProtKBacc.no.Q9BZM6(Gly26-Gly216).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
MKHHHHHHASGWVDTHCLCYDFIITPKSRPEPQWCEVQGLVDERPFLHYDCVNHKAKAFASLGKKVNVTKTWEEQTETLRDVVDFLKGQLLDIQVENLIPIEPLTLQARMSCEHEAHGHGRGSWQFLFNGQKFLLFDSNNRKWTALHPGAKKMTEKWEKNRDVTMFFQKISLGDCKMWLEEFLMYWEQMLDPTKPPSLAPG
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis:> 90%
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLin20mMTrisbuffer,50 mMNaCl,pH 8.6
Reconstitution
Add200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
