Type
Recombinant
Description
Total426 AA.MW45.7 kDa(calculated),C-TerminalFlag-tag(11 extraAA).UniProtentryQ6UXB8.
AminoAcidSequence
LTDEEKRLMVELHNLYRAQVSPPASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSLDEEPVTFPKSTHVPIPKSADKVTDKTKVPSRSPENSLDPKMSLTGARELLPHAQEEAEAEAELPPSSEVLASVFPAQDKPGELQATLDHTGHTSSKSLPNFPNTSATANATGGRALALQSSLPGAEGPDKPSVVSGLNSGPGAAADYKDDDDK
Source
HEK293
Purity
Purityasdeterminedbydensitometricimageanalysis > 99%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanPeptidaseinhibitor16
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,5 μg/lane
3.non-reducedandnon-boiledsample,5 μg/ lane
Endotoxin
<0.1 EU/µg
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
