Type

Recombinant

Description

Total247AA.MW:25.9kDa(calculated).UniProtKBacc.no.Q9NP55(Gln20–Val256).N-terminalHis-tag(10extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASQFGGLPVPLDQTLPLNVNPALPLSPTGLAGSLTNALSNGLLSGGLLGILENLPLLDILKPGGGTSGGLLGGLLGKVTSVIPGLNNIIDIKVTDPQLLELGLVQSPDGHRLYVTIPLGIKLQVNTPLVGASLLRLAVKLDITAEILAVRDKQERIHLVLGDCTHSPGSLQISLLDGLGPLPIQGLLDSLTGILNKVLPELVQGNVCPLVNEVLRGLDITLVHDIVNMLIHGLQFVIKV

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:>95%

SDS-PAGEGel

14%SDS-PAGEseparationofHumanPLUNC:

1. M.W.Marker–14,21,31,45,66,97kDa
2. Reducedandboiledsample,2.5μg/lane
3. Non-reducedandnon-boiledsample,2.5μg/lane

Endotoxin

<0.1 EU/µg

Formulation

Filtered(0.4μm)andlyophilizedfrom0.5mg/mlsolutionin20mMTrisbuffer,50mMNaCl,5%w/vtrehalosa,pH7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.