Type
Recombinant
Description
CD34isahighlyglycosylatedtypeI membraneproteinthatisselectivelyexpressedonhematopoieticstemcellsandvascularendothelium.IthasbeenwidelyusedasamolecularMarkerfortheidentification,isolation,andmanipulationofhemopoieticstemcellsandprogenitors.CD34canfunctionasaregulatorofhemopoieticcelladhesionbymediatingtheattachmentofstemcellstobonemarrowstromalcellsorotherbonemarrowcomponents.ThefulllengthhumanCD34isa385 aminoacidprotein,consistingofa31 aminoacidsignalsequence,a74 aminoacidcytoplasmicdomain,a21 aminoacidtransmembranedomainanda259 aminoacidextracellulardomain.RecombinantHumansCD34isa258 aminoacidpolypeptidecontainingonlytheextracellulardomainofthefulllengthCD34protein.ThecalculatedmolecularweightofRecombinantHumansCD34is27.4 kDa.
AminoAcidSequence
LDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKT
Source
ChineseHamsterOvaryCells(CHO)
Purity
95%
BIOLOGicalActivity
Datanotavailable.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C