Type
Recombinant
Description
VEGF-B,amemberoftheVEGFfamily,isapotentgrowthandangiogeniccytokine.ItpromotesDNAsynthesisinendothelialcells,helpsregulateangiogenesisandvascularpermeABIlity,andinhibitsapoptosisincertainsmoothmusclecellsandneurons.VEGF-Bisexpressedinalltissuesexcepttheliver.Itformscellsurface-associated,disulfide-linkedhomodimers,andcanformheterodimerswithVEGF-A.Therearetwoknownisoforms,formedbyalternativesplicing,whichhavebeendesignatedVEGF-B167andVEGF-B186.Bothformshaveidenticalamino-terminalsequencesencodingacysteineknot-likestructuralmotif,butdifferintheircarboxyl-terminaldomains.BothVEGF-BisoformssignalonlythroughtheVEGFR1receptor.RecombinantHumanVEGF-Bisa38.0 kDa,disulfide-linkedhomodimericproteinconsistingoftwo167 aminoacidpolypeptidechains.
AminoAcidSequence
PVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDSPRPLCPRCTQHHQRPDPRTCRCRCRRRSFLRCQGRGLELNPDTCRCRKLRR
Source
E.coli
Purity
98%
BIOLOGicalActivity
Determinedbythedose-dependentstimulationoftheproliferationofhumanumbilicalveinendothelialcells(HUVEC)inthepresenceofhumanVEGF165. TheexpectedED50forthiseffectis1.0–2.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
