Type
Recombinant
Description
Total839 AA.MW:94.0 kDa(calculated).UniProtKBacc.No.Q9H1B5(Gly37-Arg865,lumenaldomain,isoform1,naturalvariantwithThr305).N-terminallinker(2 extraAA),C-terminallinker(2 extraAA)andC-terminalHis-tag(6 extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
ASGLEEDEAGEKGRQRKPRPLDPGEGSKDTDSSAGRRGSTGRRHGRWRGRAESPGVPVAKVVRAVTSRQRASRRVPPAPPPEAPGRQNLSGAAAGEALVGAAGFPPHGDTGSVEGAPQPTDNGFTPKCEIVGKDALSALARASTKQCQQEIANVVCLHQAGSLMPKAVPRHCQLTGKMSPGIQWDESQAQQPMDGPPVRIAYMLVVHGRAIRQLKRLLKAVYHEQHFFYIHVDKRSDYLHREVVELAQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYPTRTNEELVAFLSKNRDKNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAGIVVDGGSDWFVLTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAESFFHTVLENSLACETLVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFLRLQQVSRPTFFARKFESTVNQEVLEILDFHLYGSYPPGTPALKAYWENTYDAADGPSGLSDVMLTAYTAFARLSLHHAATAAPPMGTPLCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMWLMPQGSLKLLGRSDQASRLQSLEVGTDWDPKERLFRNFGGLLGPLDEPVAVQRWARGPNLTATVVWIDPTYVVATSYDITVDTETEVTQYKPPLSRPLRPGPWTVRLLQFWEPLGETRFLVLPLTFNRKLPLRKDDASWLHAGPPHNEYMEQSFQGLSSILNLPQPELAEEAAQRHTQLTGPALEAWTDRELSSFWSVAGLCAIGPSPCPSLEPCRLTSWSSLSPDPKSELGPVKADGRLRKLHHHHHH
Source
HEK293
Purity
Purityasdeterminedbydensitometricimageanalysis: >95%
SDS-PAGEGel
14 %SDS-PAGEseparationofHumanXylosyltransferase2:
1. MWMarker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5 μg/lane
3.non-reducedandnon-boiledsample,2.5 μg/lane
Endotoxin
<0.1 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Filteryourculturemedia/workingsolutionscontainingthisproductbeforeusingincellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cforthree days.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
