The non-cleavable 6xHis-tagged linear Ub6 is resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind linear polyUb chains. After binding, 6XHis-tagged non-cleavable linear Ub6 and the binding proteins can be enriched with Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with TEV protease (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
Additional Information
| Product Name: | Linear polyUb Chain-binding Protein Identification / Validation Kit |
|---|---|
| Also Known As: | N/A |
| Catalog No.: | J2520 |
| Size: | 1 Kit |
| Molecular Weight: | N/A |
| Species: | Human |
| Source: | Bacterial recombinant |
| Stock: | 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol |
| Concentration: | See tube label |
| Quality Assurance: | 95% based on SDS-PAGE |
| Storage: | See the storage condition on the label of each component; avoid multiple freeze-thaw cycles |
| PDF Data Sheet: | PDF datasheet |
| NCBI RefSeq: | N/A |
| Image(s): | No |
| Shipping Method: | Dry ice shipping |
| References: | N/A |
Details
* This kit is designed for two purposes: 1) identification of proteins that specially bind linear polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between linear polyUb chains and their binding proteins.
Description
The non-cleavable 6xHis-tagged linear Ub6 is resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind linear polyUb chains. After binding, 6XHis-tagged non-cleavable linear Ub6 and the binding proteins can be enriched with Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with TEV protease (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
Components
• 6xHis-Non-cleavable Linear Ub 6 [Store at -80°C]..........100 µg
• 6xHis-Ubiquitin [Store at -80°C].............................................................................100 µg
• Nickel XPure Agarose Resin (suspended in 50% slurry) [Store at 2-8°C]....................500 µl
• 4 M Imidazole [Store at -80°C]......................................................................................500 µl
• Iodoacetamide ( IAA ) [Store at -80°C]..........................................................................25 mg
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植物：抗虫、抗病、抗除草剂植物培育、改良作物品质（富含赖氨酸的玉米）
动物：提高生长速度、生产药物蛋白、器官移植、乳腺生物反应器
微生物：疫苗
基因治疗等等
各位业内前辈，我们正在考虑引进符合GMP认证标准的CHO细胞系，用于表达可做疫苗生产的重组蛋白类。目前已有符合标准信息的是Thermofisher的CHO-S悬浮培养细胞资料，希望能再多了解一些和这株细胞类似的其他公司符合GMP标准的生产株细胞做个比较。谢谢指教！
而没答案
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给我提供答案
考研细胞的题答案
生物合成人胰岛素注射液，比如诺和灵R笔芯。是单一品种、不是混合的胰岛素。是不含鱼精蛋白的短效胰岛素。
