- Description
- Additional
Description
Details
RACTIVITY | Human |
SENSITIVITY | <3 ng/mL |
ASSAY RANGE | 3-400 ng/mL |
REAGENTS PROVIDED | HAMAMICROTITER PLATEHRP CONJUGATEHAMA STANDARDCALIBRATOR DILUENTWASH BUFFER (20X)SUBSTRATE ASUBSTRATE BSTOP SOLUTION |
INTENDED USE
This HAMA ELISA is to be used for the quantitative determination of human anti-mouse antibody (HAMA) in serum. It is useful for the prognosis, monitoring and follow-up patients treated with mouse IgG. This kit is intended for LABORTAROY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.
INTRODUCTION
Human Anti-Mouse Antibody (HAMA) are antibodies found in human serum which have the ability to bind to mouse immunoglobulin G (IgG). The presence of HAMA is the result of an immune reaction following an exposure to mice or other similar agent, which was able to induce the immune system to generate an antibody able to bind to mouse IgG. HAMA is commonly found in patients following treated with mouse monoclonal antibodies associated with some therapeutic or diagnostic procedures. Some human auto-antibodies, the most common of which is rheumatoid factor (Rf), by virtue of their cross reactivity are able to bind mouse IgG. The HAMA assay measures HAMA across a wide dynamic range and it is not necessary to predilute human serum samples before the HAMA ELISA testing.
PRINCIPLE OF THE ASSAY
This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRP-labelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples.
This ELISA Kit includes a Calibration Diluent for preparing a 2-fold dilution of Standard series. It allows the operator to produce a standard curve with Optical Density (O.D) versus Human Anti-Mouse Antibody concentration (ng/mL). The concentration of Human Anti-Mouse Antibody in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Additional
Additional Information
| Product Specificity | HAMA ELISA Kit |
|---|---|
| Application | Refer to Insert |
| Size | 96 wells |
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通量PARP/凋亡分析试剂盒可广泛应用于1）检测原代、肿瘤等细胞的PARP活性；2）检测凋亡前后的PARP活性；3）利用细胞裂解液筛选PARP抑制剂。
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步骤简单——探针温育、柱分离、板杂交和HRP检测
无需贵重仪器——无需如Luminex那样的贵重仪器
B.原理：
干细胞转录因子活性多重检测阵列试剂用于同时检测多种TF活性。该技术中，基于TFDNA结合位点的一致性序列，制备一系列生物素标记的探针。当探针混合物与核提取物一起温育时，每个探针寻找相应的TF，形成TF/探针复合物，通过柱离心纯化可以很容易与游离探针分开。结合的探针从混合物中分离出来，通过板杂交分析。板孔中预包被上与探针互补的特异序列，捕获的DNA探针进一步用链酶亲和素-HRP检测，化学发光检测仪测定发光强度（RLUs）。
