| Allergen: | rAra h 8 (Arachis hypogaea allergen 8.0101) |
| Unit: | 250µg |
| Source: | Pichia pastoris |
| Mol. Wt: | 17kD |
| Purification: | Purified from Pichia pastoris culture by HPLC gel filtration. Purity > 95 % by silver stained SDS-PAGE. |
| Concentration: | See product insert. |
| Formulation: | Preservative-free and carrier-free in phosphatebuffered saline, pH 7.4. Steriled filtered. |
| Storage: | Store at -20ºC. |
| Notes: | Avoid repeated freeze-thaw cycles. |
| Genbank: | |
| PDB: | |
| Product Resources: | Recombinant Ara h 8 Certificate of Analysis |
| Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. | |
| References: | |
Mittag D et al: Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J Allergy Clin Immunol. 2004, 114(6):1410-7. | |
Riecken S et al. Purification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform. Biol Chem. 2008; 389(4):415-23. | |
Asarnoj A. Peanut component Ara h 8 sensitization and tolerance to peanut. J Allergy Clin Immunol. 2012;130(2):468-72. | |
Hurlburt BK. Structure and function of the peanut panallergen Ara h 8. J Biol Chem. 2013 27; 288 (52):36890-901. | |
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本是小菜鸟,目前研究某个比较新的蛋白对内质网应激的影响,需要抑制这个蛋白的表达看下游信号的表达情况,但是无论如何夜找不到抑制剂,做RNA干扰的话经费就超出预算了!急求各位大神,帮忙出个解决方案吧!非常感谢!
作为一种 RNA 导向的 dsDNA 结合蛋白,Cas9 效应物核酸酶是已知的第一个统一因子(unifying factor),能够共定位 RNA、DNA 和蛋白,从而拥有巨大的改造潜力。将蛋白与无核酸酶的 Cas9( Cas9 nuclease-null)融合,并表达适当的 sgRNA ,可靶定任何 dsDNA 序列,而 sgRNA 的末端可连接到目标DNA,不影响 Cas9 的结合。因此,Cas9 能在任何 dsDNA 序列处带来任何融合蛋白及 RNA,这为生物体的研究和改造带来巨大潜力。向左转|向右转
http://www.addgene.org/crispr/guide/
请有经验的战友指点,若可以最好能提供几篇应用动物进行RNA干扰研究的文章.
非常感谢!
http://www.nature.com/nature/journal/v520/n7546/full/nature14299.html

