ObtainhighyieldsofqualityDNAfromyeastwiththiseasytousekit
Fast:ObtainpurifiedDNAinlessthan40minutes- FlexIBLe:PurifyDNAfromawidevarietyofyeastspecies,includingCandida,Saccharomyces,Pichia,andSchizosaccharomyces,andfilamentousfungisuchasAspergillus2andPenicillium
- HighQualityDNA:PurifyhighmolecularweightgenomicDNAthatisreadytouseinmanymolecularBIOLOGyapplications,includingPCRamplification,restrictionendonucleasedigestion,Southernblotting,genomiclibrarypreparationandfungalidentificationandtyping
- HighYields:PurifymoreyeastgenomicDNAthanothercommerciallyavailablekits
- Simple:Noenzymaticlysis,beadbeating,columns,phenolorotherorganicsolventsareused
TheMasterPure™YeastDNAPurificationKitenablesefficient,high-yieldpurificationofhigh-molecular-weightDNAfromyeastandotherfungi.Theprotocolinvolvesnonenzymaticcelllysisat65°C,followedbyremovalofproteinbyprecipitation,andnucleicacidprecipitationandresUSPension.Nolyticase,proteolyticenzymes,orbead-beatingareusedintheprocedure.YeastgenomicDNAyieldsusingtheMasterPureKitaremuchhigherthanyieldsobtainedwithothercommerciallyavailablekits(Fig.1).1Theprotocolcanbeeasilyadjustedforlargerorsmallersamples,includingsingleyeastcolonies.1Therecoverednucleicacidcanbeuseddirectlyinmostapplications,includingPCRamplification.
References
- Hoffman,L.andMoan,E.(1998)EpicentreForum5(4),1.
- Jin,J.,Lee,Y.-K.andWickes,B.L.(2004)J.ClinMicrobiol.42,4293.
| Figure1.ComparativeyieldsofDNAfromtheMasterPure™YeastDNAPurificationKitandcompetitorkits.DNAwasquantitatedspecificallywithHoechstfluorescentdye33258,whichgivesminimalfluorescencewithRNA.ThedatarepresenttheaverageDNAyieldsdeterminedbyfluorometryfromtwoexperimentswithS.cerevisiaeandC.albicans.TheMasterPureKitproducedupto17timesmoreDNAfromC.albicansand12timesmoreDNAfromS.cerevisiaethanotherkits. |
![]() | Figure2.SizedistributionofDNAproducedfromtheMasterPure™Kit.Foreachkitindicated,a500-ngaliquotofpurifiedyeastDNAwasanalyzedbypulse-fieldgelelectrophoresisona1%agarosegel.Thegelwasstainedwithethidiumbromide.ThemajorityofDNAisolatedwiththeMasterPureKitwasestimatedtobeinthe40-to50-kbsizerange,whiledegradationtosmallerfragmentswasobservedwithotherkits.LaneM,lamBDaDNAladder;Lane4,T7DNA(40kb). |
ORDERINFORMATION
Contents:YeastCellLysisSolution,MPCProteinPrecipitationReagent,TEBuffer,RNaseA.ebiomall.com
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buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.
这个与琼脂糖电泳有关。提取的DNA分子量很大,可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道,象那个一万与一万五和条带差别很近。
大家都用过哪些DNA提取试剂盒,能不能推荐几种
而且比较容易买到,同时提取的DNA便于纯化



