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RayBiotech/Human Inflammation Array Q3/8 Sample Kit/QAH-INF-3-1
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RayBiotech/Human Inflammation Array Q3/8 Sample Kit/QAH-INF-3-1
品牌 / 
RayBiotech
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QAH-INF-3-1
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数    量:
免费咨询热线
4000-520-616

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CSF1 | CSF2 View more Hide

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
BLC(CXCL13)
  • Gene Symbol: CXCL13
  • Gene ID: 10563
  • Accession #: O43927
  • ELISA Kit: RayBio Human BLC ELISA
Eotaxin-1(CCL11)
  • Gene Symbol: CCL11
  • Gene ID: 6356
  • Accession #: P51671
  • ELISA Kit: RayBio Human Eotaxin-1 ELISA
Eotaxin-2(MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Gene ID: 6369
  • Accession #: O00175
  • ELISA Kit: RayBio Human Eotaxin-2 ELISA
GCSF
  • Gene Symbol: CSF3
  • Gene ID: 1440
  • Accession #: P09919
  • ELISA Kit: RayBio Human G-CSF ELISA
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
  • ELISA Kit: RayBio Human GM-CSF ELISA
I-309(TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Gene ID: 6346
  • Accession #: P22362
  • ELISA Kit: RayBio Human I-309 ELISA
ICAM-1(CD54)
  • Gene Symbol: ICAM1
  • Gene ID: 3383
  • Accession #: P05362
  • ELISA Kit: RayBio Human sICAM-1 ELISA
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
  • ELISA Kit: RayBio Human IFN-gamma ELISA
IL-1 alpha(IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
  • ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta(IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
  • ELISA Kit: RayBio Human IL-1 beta ELISA
IL-1 ra(IL-1 F3)
  • Gene Symbol: IL1RN
  • Gene ID: 3557
  • Accession #: P18510
  • ELISA Kit: RayBio Human IL-1 RA ELISA
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
  • ELISA Kit: RayBio Human IL-2 ELISA
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
  • ELISA Kit: RayBio Human IL-4 ELISA
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
  • ELISA Kit: RayBio Human IL-5 ELISA
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
  • ELISA Kit: RayBio Human IL-6 ELISA
IL-6 R
  • Gene Symbol: IL6R
  • Gene ID: 3570
  • Accession #: P08887
  • ELISA Kit: RayBio Human IL-6 sR ELISA
IL-7
  • Gene Symbol: IL7
  • Gene ID: 3574
  • Accession #: P13232
  • ELISA Kit: RayBio Human IL-7 ELISA
IL-8(CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
  • ELISA Kit: RayBio Human IL-8 ELISA
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
  • ELISA Kit: RayBio Human IL-10 ELISA
IL-11
  • Gene Symbol: IL11
  • Gene ID: 3589
  • Accession #: P20809
  • ELISA Kit: RayBio Human IL-11 ELISA
IL-12 p40
  • Gene Symbol: IL12B
  • Gene ID: 3593
  • Accession #: P29460
  • ELISA Kit: RayBio Human IL-12 p40 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
  • ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
  • ELISA Kit: RayBio Human IL-13 ELISA
IL-15
  • Gene Symbol: IL15
  • Gene ID: 3600
  • Accession #: P40933
  • ELISA Kit: RayBio Human IL-15 ELISA
IL-16
  • Gene Symbol: IL16
  • Gene ID: 3603
  • Accession #: Q14005
  • ELISA Kit: RayBio Human IL-16 ELISA
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
MCP-1(CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
  • ELISA Kit: RayBio Human MCP-1 ELISA
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #: P09603
  • ELISA Kit: RayBio Human M-CSF ELISA
MIG(CXCL9)
  • Gene Symbol: CXCL9
  • Gene ID: 4283
  • Accession #: Q07325
  • ELISA Kit: RayBio Human MIG ELISA
MIP-1 alpha(CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
  • ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta(CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
  • ELISA Kit: RayBio Human MIP-1 beta ELISA
MIP-1 delta(CCL15)
  • Gene Symbol: CCL15
  • Gene ID: 6359
  • Accession #: Q16663
  • ELISA Kit: RayBio Human MIP-1 delta ELISA
PDGF-BB
  • Gene Symbol: PDGFB
  • Gene ID: 5155
  • Accession #: P01127
  • ELISA Kit: RayBio Human PDGF-BB ELISA
RANTES(CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
  • ELISA Kit: RayBio Human RANTES ELISA
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: 7076
  • Accession #: P01033
  • ELISA Kit: RayBio Human TIMP-1 ELISA
TIMP-2
  • Gene Symbol: TIMP2
  • Gene ID: 7077
  • Accession #: P16035
  • ELISA Kit: RayBio Human TIMP-2 ELISA
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
  • ELISA Kit: RayBio Human TNF-alpha ELISA
TNF beta(TNFSF1B)
  • Gene Symbol: LTA
  • Gene ID: 4049
  • Accession #: P01374
  • ELISA Kit: RayBio Human TNF-beta ELISA
TNF RI(TNFRSF1A)
  • Gene Symbol: TNFRSF1A
  • Gene ID: 7132
  • Accession #: P19438
  • ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII(TNFRSF1B)
  • Gene Symbol: TNFRSF1B
  • Gene ID: 7133
  • Accession #: P20333
  • ELISA Kit: RayBio Human sTNFRII ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Inflammation Array Q3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Platonov M., Borovjagin AV., Kaverina N., et al. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Lett. 2018 Mar 28;417:75-88. doi: 10.1016/j.canlet.2017.12.024. Species: HumanSample type: Conditioned Media
  2. Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.Species: HumanSample type: Serum
  3. Liao Y., Ivanova L., Zhu H., et al. Cord Blood-Derived Stem Cells Suppress Fibrosis and May Prevent Malignant Progression in Recessive Dystrophic Epidermolysis Bullosa. Stem Cells. 2018 Sep 24. doi: 10.1002/stem.2907. Species: HumanSample type: Conditioned Media
  4. Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327Species: HumanSample type: Conditioned Media
  5. Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.Species: HumanSample type: Conditioned Media
  6. Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094Species: HumanSample type: Conditioned Media
  7. Wang S., Lu Y., Hong Q., et al. Protein Array-Based Detection of Proteins in Kidney Tissues from Patients with Membranous Nephropathy. Biomed Res Int. 2017; 2017: 7843584. doi: 10.1155/2017/7843584Species: HumanSample type: Tissue Lysate
  8. Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.Species: HumanSample type: Vitreous Fluid
  9. Yang B., Deng Q., Zhang W., et al. Exposure to Polycyclic Aromatic Hydrocarbons, Plasma Cytokines, and Heart Rate Variability. Sci Rep. 2016 Jan 13;6:19272. doi: 10.1038/srep19272.Species: HumanSample type: Plasma
  10. Gu R., Zhou M., Jiang C., et al. Elevated concentration of cytokines in aqueous in post-vitrectomy eyes. Clin Exp Ophthalmol. 2016 Mar;44(2):128-34. doi: 10.1111/ceo.12638Species: HumanSample type: Aqueous Humor
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My lab members have used this array several times before, they recommend it to me, both mouse's and human's are very reliable!
The Wistar Institute
It's good and easy to use, but need a laser scanner.
University of South Florida
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请问各位大神,我实验室有这些试剂盒,QIAprepSpinMiniprepKit(250)、AllPrepDNA/RNAMiniKit(50)、QIAampDNAMiniKit、WizardGenomicDNAPurificationKit,请问这几个提DNA的试剂盒有什么不同吗?
有哪些可以提取细胞中病毒DNA的?或者用其他试剂盒提过呢
坐等回复!
DNA提取试剂盒哪种好 123
沙拉曼德2016-03-14
如题,用过天根的但效果不太好,想换一种,但Qiagen的太贵,能不能推荐一个性价比高的牌子?
dna提取试剂盒说明书123
ewen71172021-07-23
**推荐性价比高的DNA提取试剂盒,提取分选后的细胞,细胞量不大。
DNA的粗提取和鉴定123
sha2842017-10-03
需要什么试剂?具体的配制方法是怎样的?要非常具体的!
主要成分是:NaCl,Tris-HCl(PH=8),EDTA(PH=8),SDS.NaCl,Tris-HCl主要是调节溶液的PH,维持一定的离子浓度。EDTA主要是二价金属离子的螯合剂,使影响DNA的DNA酶中的钙离子和镁离子和EDTA结合,使酶失去活性,有利于提取完整DNA;SDS主要是和蛋白质结合,使其变形沉淀,从而利于DNA的提取,减少和清除带白质杂质!
这个与琼脂糖电泳有关。提取的DNA分子量很大,可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道,象那个一万与一万五和条带差别很近。
dna产物纯化试剂盒可以纯化甲基化之后的dna
1、DNA提取问题:现在核算提取试剂盒大部分都是吸附柱法,相信你应该不会操作错误,如果提取之后进行核酸定量结果很低的话,试着再最后一步溶解核算的时候把水稍微加温(37℃就可以了),溶解时间稍微长一些,然后再离心的时候采用分次离心,比如开始你用200ul溶解的话,你可以分两次用100ul水溶解离心,这样可以提高DNA的产率.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
CTAB法提取DNA中各试剂的用途
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.
真菌的细胞成分和植物是有差别的,这种专一性的植物DNA提取试剂盒不一定能提好真菌的DNA.如果有条件你最好是买专一性的真菌DNA提取试剂盒.不过植物DNA提取的CTAB法确实可以适用于真菌的DNA提取,但因为真菌菌丝体含有很多多糖,你需要参考下植物DNA。
水稻和棉花的种子
PVPP是不溶于水和有机溶剂的,请问怎样配置成0.01g每ml,不胜感激
这两个方法有什么区别?那个比较好?有什么要主意的吗?

提取试剂盒的详细步骤是什么?
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