
Test Label: NATest Format: ImmunoStrip®Capture Reagent: NADetection Reagent: NA
Intended Use:
Roundup Ready® cotton expresses the selectable marker protein neomycin phosphotransferase (NPT II), while Roundup Ready® Flex cotton does not. This ImmunoStrip® was developed and validated to determine the presence or absence of NPT II protein in single seeds of Roundup Ready® cotton and to confirm the absence of the NPT II protein in single seeds of Roundup Ready® Flex cotton.This ImmunoStrip® is appropriate only when testing varieties of (Roundup Ready® cotton, which expresses the NPT II protein) and varieties of (Roundup Ready® Flex cotton, which does not express the NPTII protein). Testing any stacked-trait events, such as Bollgard II with Roundup Ready® cotton is not recommended. Only Roundup Ready® and Roundup Ready® Flex events listed are appropriate for testing. Composite testing of seeds and leaves is also not recommended.The NPT II ImmunoStrip® test will detect only the NPT II protein and has shown no cross-reaction with PAT/bar, PAT/pat, Bt-Cry1Ac, Bt-Cry2Ab or Bt-Cry1F.
Includes:
- ImmunoStrips®
- SEB4 sample buffer (seed testing), not included, see related products
- PEB1 sample buffer (leaf testing), not included, see related products
- User Guide
ebiomall.com






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这个与琼脂糖电泳有关。提取的DNA分子量很大,可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道,象那个一万与一万五和条带差别很近。
1、DNA提取问题:现在核算提取试剂盒大部分都是吸附柱法,相信你应该不会操作错误,如果提取之后进行核酸定量结果很低的话,试着再最后一步溶解核算的时候把水稍微加温(37℃就可以了),溶解时间稍微长一些,然后再离心的时候采用分次离心,比如开始你用200ul溶解的话,你可以分两次用100ul水溶解离心,这样可以提高DNA的产率.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.

