
Product Name | HiLyteᵀᴹFluor 488 hydroxylamine, HCl salt *single isomer*HiLyte Fluor 488 C2 - aminooxyacetamide, HCl salt *single isomer* |
Size | 1 mg |
Catalog # | AS-64348 |
US$ | $342 |
HiLyte Fluor 488 hydroxylamine is a carbonyl-reactive labeling dye, which reacts more readily with aldehydes at physiological pH than other primary amine-containing reagents (such as hydrazides and amines). The reaction of HiLyte Fluor 488 hydroxylamine with aldehydes yields oxime ethers, which are stable at physiological conditions. Therefore, HiLyte Fluor 488 hydroxylamine can be used to directly detect aldehydes in proteins and DNA.Extinction Coefficient (M-1cm-1): 70,000Fluorescence quantum yield: 0.91Fluorescence Life Time (ns): 4.1 | |
Detailed Information | ![]() |
Storage | -20°C desiccated and protected from light |
References | D. Boturyn, et al, Tetrahedron, 53, 5485 (1997); J. G. Hecker, et al, ChemmedChem, 3, 1356 (2008); Y. Zeng, et al, Nature methods, 6, 207 (2009). |
Molecular Weight | 525.94 |
Spectral Properties | Abs/Em = 498/525 nm |
Solvent System | DMF or DMSO |
Product Citations | Fabrini R. et al. Biochemistry. 2009 Oct 9 |
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这个与琼脂糖电泳有关。提取的DNA分子量很大,可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道,象那个一万与一万五和条带差别很近。
1、DNA提取问题:现在核算提取试剂盒大部分都是吸附柱法,相信你应该不会操作错误,如果提取之后进行核酸定量结果很低的话,试着再最后一步溶解核算的时候把水稍微加温(37℃就可以了),溶解时间稍微长一些,然后再离心的时候采用分次离心,比如开始你用200ul溶解的话,你可以分两次用100ul水溶解离心,这样可以提高DNA的产率.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.

