Introduction Over one third of diabetics will develop diabetic kidney disease (DKD) in their lifetime1. Despite the large amount of diabetics suffering from DKD, current methods for measuring and tracking kidney function may not be specific enough for diabetic systems2,3. […]
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Catalog #54-043SpeciesCanineRegulatory StatusResearch Use Only. Not for Use in Diagnostic Procedures.Product DistributionAvailable in North America OnlyRange4 - 400 pg/mLSensitivity0.56 pg/mLSizes96 WellsSample TypesCell Culture, Plasma, Serum, Tissue Culture, UrineIncubation Time3 hrIncubation Time3 hr 10 minSample Size10µLInstructions for UseClick here to viewSafety Data SheetClick here to view - Additional Information
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NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are small secreted proteins characterized by their ability i) to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, ii) to bind to specific cell-surface receptors, and iii) to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin2 (LCN 2); more recently the name siderocalin has been used to express NGAL’s ability to bind bacterial siderophores. As NGAL was first reported as oncogene 24p3 in the mouse, this name is still occasionally used to identify NGAL in other species.Because dog NGAL has not yet been isolated and no antibodies or immunochemical detection methods have hitherto been developed to detect or measure it, there are no published data on the physiological or pathological functions of this protein in the dog. However, it is likely that some features of the biosynthesis, location and release of dog NGAL will be similar to those of its homologues in other species. The present kit has in fact been developed to extend NGAL research and the use of NGAL as a marker molecule, especially for kidney injury, to the dog. In the following, some pathophysiological features of NGAL in other mammalian species will be mentioned, because similar features may be observed in the dog.Like other mammalian NGALs, dog NGAL may in certain situations be co-expressed and form complexes with matrix metalloproteinase-9 (MMP-9). Dog NGAL may also prove to form homodimers and higher oligomers with itself. As dog NGAL, unlike its human homologue but like all other mammalian NGALs known, does not possess a third cysteinyl residue capable of forming an intermolecular disulfide bridge, any complex formation detected will probably depend on non-covalent forces, at least initially.
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DNA聚合酶和DNA连接酶作用的位点都是3'5'磷酸二酯键;但DNA连接酶是作用在游离的DNA片段间,使其连接成为一条完整的DNA链,而DNA聚合酶则是将游离的脱氧核糖核苷酸连接成DNA片段。
最近,我看了很多这方面的资料,但是我对这个方面不是很了解,所以现在搞得头昏眼花的,不知道该选什么酶和载体,希望大家多多帮忙
在此十分感谢
解旋酶:在DNA复制、转录时,作用于DNA双链,将双链DNA解开形成单链。
RNA聚合酶:在转录过程中,作用于游离的核糖核苷酸,将它们连接形成mRNA链
聚合酶就是多功能的dna的制作机器
但是也有以RNA为模板的DNA聚合酶和RNA聚合酶 (也就是反转录酶和RNA依赖的RNA聚合酶),它们的结合位点在RNA上.
也就是说,模版是谁,结合位点就在谁上。
2.结合位点就是启动合成DNA或者RNA的碱基序列,如RNA聚合酶结合位点是转录起始位点,是一段特殊的位于编码基因上游的DNA序列.这段DNA序列可以结合RNA聚合酶,从而起始转录过程.经典的RNA聚合酶结合位点是TATA box.
TATA box是编码序列前的4个碱基.在大多数生物的基因前面都有TATA着4个碱基序列的存在,它就是一个RNA聚合酶结合位点.
再来说真核生物,真核生物的DNA聚合酶分为α,β,γ,δ,ε,.首先我来说三个和原核生物中的Ⅰ,Ⅱ,Ⅲ功能相同的酶.ε类似于原核生物DNA聚合酶Ⅰ,β类似于原核生物DNA聚合酶Ⅱ,δ类似于原核生物DNA聚合酶Ⅲ.α具有引物酶活性,而γ则是作为复制真核生物线粒体内的DNA所需要的酶.


