DescriptionMoloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA dependent DNA polymerase. It can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The absence of RNase H activities enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.
Concentrations 100 - 500u/μl
Assay Conditions 50mM Tris- HCl (pH8.3), 6mM MgCl2, 10mM DTT, 0.4mM Poly(rA)•(dT)12-18, and 0.5mM [ 3H]dTTPin a reaction volume of 50μl./μl
Supplied With10X Buffer M-MuLV RT 500mM Tris-HCl (pH 8.3 at 25˚C), 75mM KCl, 3mM MgCl2 and 10mM DTT. Store at -20˚C.
Storage Buffer 10mM K-Phosphate (pH 7.5), 0.1mM EDTA, 200mM NaCl, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20˚C.
Thermal Inactivation 70˚C for 10 minutes
Unit Definition 1u is defined as the amount of enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•oligo(dT).
Application
- First strand cDNA synthesis.
- DNA labeling.
- RNA analysis by primer extension.
Quality ControlPurified free of contaminating endonucleases and exonucleases.
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| ME2305 | M-MuLV Reverse Transcriptase (RNase H¯) | 10000u |
| ME2306 | M-MuLV Reverse Transcriptase (RNase H¯) | 50000u |
DownloadManual
M-MuLV Reverse Transcriptase (RNase H¯)
PublicationThis Product Has Been Used In:(2020)Detection of Tomato Mosaic Virus Viruses in Farms and Greenhouses and Breed Several Isolates of The Potato Virus Y From this Host International Journal of Advanced Science and Technology, 29(8s):1650-1655.Faezebossadat A & Seyyedeh AH (2020)Incidence and distribution of Tobacco streak virus isolated from parsley Petroselinum sativum, Journal of Crop Protection, 9(4): 683-697.Mirzaei et al. (2019) Synergistic effects of polyaniline and pulsed electromagnetic field to stem cells osteogenic differentiation on polyvinylidene fluoride scaffold, . Artificial Cells, Nanomedicine and Biotechnology,47(1):3058-3066. Moradi, N. et al. (2019) Plasma Level of miR-5193 as a Novel Biomarker for Diagnosis of HBV-Related Hepatocellular Carcinoma, . Hepatitis Monthly, 19(2):e84455.Kaplan, S. et al. (2016) The Potential of Microarray Databases to Identify Tissue Specific Genes Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(1), p. 29-35. Noureini, S.K., Tanavar, F. (2015) Boldine, a natural aporphine alkaloid, inhibits telomerase at non-toxic concentrations. Chemico-Biological Interactions. 231. Pp27-34. Tahoori, M.T., Pourfathollah, A.A., Soleimani, M., Vasheghani-Farahani, E., Mohammadzadeh, A., Amari, A., Hashemi, S.M., mossahebi-mohammadi, M. (2015) Fibroblasts feeder niche and Flt3 Ligand as a novel inducer of plasmacytoid dendritic cells development in vitro. International Immunopharmacology. 24. Pp.474-480.Ahmadi, E., Ahmadi, M., PourBahsh, S.A., Talebi, A. (2013). Detection and Differentiation of Newcastle Disease Virus Strains Affecting Commercial Poultry in Northwest of Iran using RT-PCR, International Journal of Veterinary Science, Vol 2, No. 4, 138-142. Supathaweewat, K., Klanrit, P (2013). cDNA cloning and expression analyses of phytoene synthase 1, phytoene desaturase and -carotene desaturase genes from Solanum lycopersicum KKU-T34003, Songklankarin Journal of Science and Technology, Vol. 35, No. 5, 517-527 (2013).
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
配后是否应该分装,因为不能反复冻融?
多谢!
1、发帖请归入相应子版（选择SubBoard）！
2、请在帖子前面注明帖子性质，如【求助】、【原创】、【分享】、【讨论】、【申请上传】、【下载】、【转帖】、【转载】、【信息】、【求购】、【公告】、【建议】等，以方便战友应助及版面管理！
3、不规范的发帖及应助帖将不加分！
4、对于得分在5分以下或贴子数少于20的战友的不规范发贴，第一次我们将帮助修改同时给予提示；对于5分以上或贴子数大于20的战友的不规范发贴将直接锁贴，等待修改（可PM版主），保留24h仍然不修改，将删除所发的贴子而且不另外通知！
【】可以这样输入，智能ABC，输入“v1”，后翻3页，就可以看见了，或用紫光拼音中文输入法，输入方括号就是粗体的中括号，实在不行请复制本公告中【】
我做的双酶切反应，酶分别是宝生物的BamHI和XhoI，双酶切体系是：载体及目的片段分别是30ul，通用buffer4ul，XhoI、BamHI各2ul，无核酶水2ul，总体积40ul。载体和目的片段都是经37度酶切6h。载体上面这两个酶切位点的距离是6个碱基，目的片段是由pcr反应获得的，从puc19中p出来的，两端带有这两种酶的酶切位点，酶切完成后，做连接反应，体系如下：目的片段（全长1441bp）4ul，无核酶水3ul，10*T4DNA连接酶缓冲液1ul，载体1ul，T4DNA连接酶1ul，总体积10ul。16度过夜。之后摇菌送沉菌测序结果回来，送了5管一个都不对。重复实验两次还是不对！
之后改为先用BanHI单切载体，体系如下：载体17ul，酶1ul，buffer2ul，总体积20ul，30度切6小时后，Promega纯化试剂盒直接纯化，完了之后再用XhoI，37度酶切6小时，体系如上，再次用promega纯化试剂盒纯化，目的片段用双酶切体系酶切，之后做连接反应，之后铺板，挑取菌落共10个，摇菌13小时，取菌液做pcr鉴定，结果10个菌落全是引物2聚体，跑出来的条带都是100bp左右。
各位老师这是怎么回事呢？为什么连接不上呢？小弟先谢过各位大哥了！
求问酶切体系为10ul,内切酶1ul,37℃水浴45min，不知道DNA产物是否完全被切开了？
所用内切酶信息如下
同一管dna有的酶可以切开，比如dra1，ecoR1,Hind111
有的酶又完全不能切动，如BamH1，sac1，xba1，xho1，pst1
请问高手这是什么原因？?
感谢赐教~！
