| Catalog number | LS162C |
| Product type | ELISA |
| Quantity | 96 Tests (12x8 breakable strip wells) |
| Standard range | 1-40 ng/ml |
| Analytical Sensitivity | 1 ng/ml |
| Sample volume | 25 µl/well |
| Species | Human |
| Storage and Stability | Product should be stored at 2-8 °C. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Agharanya JC. Clinical usefulness of ELISA technique in the assessment of thyroid function. West Afr J Med 1990;9(4):258-63. 2. Hankiewiz, J. and Swierczuk, E. 1974. Lysozymes in Human Body Fluids. Clinica Chemica Acta, 57: 205-209. 3. Meyor, K., Gelhorn, A., Prudden, J.F., et al. 1948. Lysozyme Activity in Ulcerative Alimentary Diseases. American Journal of Medicine, 5: 496-502. 4. Prockup, D.J. and Davidson, W.D., 1964. A Study of Urinary and Serum Lysozyme in Patients with Renal Disease, New England Journal of Medicine, 270: 269. |
Lysozyme ELISA Package InsertType : PDF
Alpha 1-Antichymotrypsin (ACT) ELISA

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Bone Mesenchymal Stem Cells 作为一个细胞群体,还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法,就是采用培养,colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数,比如STRO-1,但是一般认为STRO-1阳性的细胞更趋向于造血干细胞,和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注,图注的意思我明白,但是我想知道merge后的图看什么颜色的荧光,蓝色是细胞核,红色是lysosome(位于胞质),绿色是AMPKβ2,该实验是想观察AMPKβ2是否转位到lysosome上了,如果确实发生了AMPKβ2转位到lysosome上,那么merge后是红色与绿色融合在一起,是吗?融合在一起发什么颜色的光了?

