- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody against Myc tag
Product Type:Purified primary antibodies
Target Protein:Myc tagged recombinant protein
Immunogen:A synthetic peptide (EQKLISEEDL) corresponds to amino acids 410-419 of transcription factor c-Myc. Coupled to KLH for immunization.
Fusion Myeloma:Sp2/0-Ag14
Specificity:This monoclonal antibody binds to Myc-tagged recombinant protein.
Cross-Reacitvity:Not identified
Host / Isotype: Mouse, IgG1 Kappa
Formulation:Lyophilized from a solution in pH 7.2. 0.01M PBS with 0.05% sodium azide.
Storage:Aliquot and store at -20 oC for long term storage. Avoid repeated freeze and thaw cycles.
Research Area:Life science
Application:
Western blotting: 1:1,000

Fig. Westen Blot analysis of Myc-tagged fusion construct under different conditions of transfection reagents and amount of fusion DNA construct.
Immunoprecipitation and Immunostaining: 1:200
Citations
1. Li HY, Yeh PA, Chiu HC, Tang CY, Tu BP. Hyperphosphorylation as a defense mechanism to reduce TDP-43 aggregation. PLoS One 2011; 8(6):e23075.
Additional
Additional Information
| Product Specificity | mAb anti-Myc Tag, MycA7 |
|---|---|
| Application | WB, IHC |
| Size | 0.1 mg |
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本人对软骨石蜡切片做了MMP-13的免疫组化染色,首次做,无法判断染色部位,请各位帮忙看一下
最近开始做TUNEL染色,第一次按罗氏protocol做的,阴性对照是没有显色的。没做阳性对照。第二次、第三次做出来背景都很深,肾小管每个细胞核上都染上了棕黄色,DAB染色很快,几秒就变一片黄。具体步骤是:1烤片脱蜡至水2.蛋白酶K20ug/ML37℃10分钟3.tunel液冰上配置后加组织上,37度1h。4.pod液37℃30min5.DAB显色。在蚂蚁淘中检索后,优化了实验条件,在蛋白酶K前加了3%H2O2封闭10min,缩短了tunel液至40min。这是第三次的结果大家帮我看看
这是对照组
这是模型组

