Description
Product Highlights
- Fast – single-tube protocol that eliminates wash steps, giving high-yield, PCR-ready DNA in just 15 minutes
- Simple – few protocol steps greatly reduce the risk of sample loss and contamination and minimizes manual effort
- Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targets
- Specific - MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samples
- Convenient – mastermix facilitates PCR set-up and includes a red dye for improved pipetting ease / accuracy and to enable direct gel loading
Product Description
Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination.
The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Meridian. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield.
The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization
Applications
- High-throughput genotyping from mammalian tissue
- Detection of transgenes
- Knockout analysis
Introduction to MyTaq
Overview, features and benefits of the MyTaq product familyPCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchApplication Note
MyTaq™ Extract-PCR Kitebiomall.com
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Bone Mesenchymal Stem Cells 作为一个细胞群体，还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法，就是采用培养，colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数，比如STRO-1，但是一般认为STRO-1阳性的细胞更趋向于造血干细胞，和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注，图注的意思我明白，但是我想知道merge后的图看什么颜色的荧光，蓝色是细胞核，红色是lysosome(位于胞质），绿色是AMPKβ2，该实验是想观察AMPKβ2是否转位到lysosome上了，如果确实发生了AMPKβ2转位到lysosome上，那么merge后是红色与绿色融合在一起，是吗？融合在一起发什么颜色的光了？
