| Product Name | [Leu31, Pro34] - Neuropeptide Y, human, ratYPSKPDNPGEDAPAEDMARYYSALRHYINLLTRPRY - NH2 |
| Size | 1 mg |
| Catalog # | AS-24366 |
| US$ | $186 |
| Purity | % Peak Area By HPLC ≥ 95% |
Like neuropeptide Y (NPY) and other peptides of the family, this peptide adopts a folded hairpin structure with the terminal segments in close proximity.An intracerebroventricular injection of NPY or [Leu31, Pro34]-NPY (non-Y2 receptor agonist) given during middle cerebral artery occlusion increases the infarct volume and nitric oxide (NO) overproduction in the rat brain. | |
| Detailed Information | |
| Storage | -20°C |
| References | Ref: Futilendorf, I. et al. Proc. Natl. Acad. Sci. USA 87, 182 (1990). Khiat, AJ. Pept. Res. 51, 317 (1998); Chen, S. and RJ. Cheung, Biomed. Sci.12, 267 (2005); Chen, S. and RJ. Cheung, Biomed. Sci. 11, 781 (2004); Labelle, M. et al. Peptides 18, 801 (1997) |
| Molecular Weight | 4240.8 |
| YPSKPDNPGEDAPAEDMARYYSALRHYINLLTRPRY-NH2 | |
| Sequence(Three-Letter Code) | H - Tyr - Pro - Ser - Lys - Pro - Asp - Asn - Pro - Gly - Glu - Asp - Ala - Pro - Ala - Glu - Asp - Met - Ala - Arg - Tyr - Tyr - Ser - Ala - Leu - Arg - His - Tyr - Ile - Asn - Leu - Leu - Thr - Arg - Pro - Arg - Tyr - NH2 |
| Product Citations | Dimitrov, EL. et al. Endocrinology 148, 3666 (2007).Kapoor, JR. and CD. Sladek, Am J Physiol Regul Integr Comp Physiol 280, R69 (2001). |
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Bone Mesenchymal Stem Cells 作为一个细胞群体,还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法,就是采用培养,colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数,比如STRO-1,但是一般认为STRO-1阳性的细胞更趋向于造血干细胞,和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注,图注的意思我明白,但是我想知道merge后的图看什么颜色的荧光,蓝色是细胞核,红色是lysosome(位于胞质),绿色是AMPKβ2,该实验是想观察AMPKβ2是否转位到lysosome上了,如果确实发生了AMPKβ2转位到lysosome上,那么merge后是红色与绿色融合在一起,是吗?融合在一起发什么颜色的光了?

