| Product Name | Lymphocytic Choreomeningitis Virus (LCMV) Glycoprotein 33 (33 - 41), LCMV GP1, gp 33 (33 - 41)KAVYNFATC |
| Size | 1 mg |
| Catalog # | AS-61669 |
| US$ | $108 |
This is the H-2Db restricted epitope derived from the Lymphocytic Choreomeningitis Virus (LCMV) glycoprotein gp 33; residues 33 to 41. | |
| Detailed Information | |
| Storage | -20°C |
| References | Oldstone, M. et al. J. Virol. 69, 7423 (1995); Zhou, S. et al. J. Virol. 78, 3578 (2004); Karrer, U. et al. J. Virol. 78, 2255 (2004). |
| Molecular Weight | 1016.2 |
| KAVYNFATC | |
| Sequence(Three-Letter Code) | H - Lys - Ala - Val - Tyr - Asn - Phe - Ala - Thr - Cys - OH |
| Product Citations | Towne, C.F. et al. (2008). Puromycin-Sensitive Aminopeptidase Limits MHC Class I Presentation in Dendritic Cells but Does Not Affect CD8 T Cell Responses during Viral Infections. J Immunol 180, 1704. |
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Bone Mesenchymal Stem Cells 作为一个细胞群体,还没有发现有特定细胞表面marker. 对于那些可以代表自我更新和分化的marker, 也不清楚到底要发现哪一个的表达才能确定该细胞就是BMSC。
目前常用的方法,就是采用培养,colony-forming unit-fibroblasts (CFU-F)这个方法。一般BMSC可以24-48小时贴壁。
流式细胞计数,比如STRO-1,但是一般认为STRO-1阳性的细胞更趋向于造血干细胞,和BMSC简单区别还不是很清楚。
这里有个培养分化的产品
http://www.rndsystems.com/pdf/SC020.pdf
GlucosestarvationcausestranslocationofAMPKβ2tothelysosomeinHEK-293cellsthatisdependentonN-myristoylation.Theexperimentwasperformedinβ2KOcellsasinFig.1c,exceptthatthelysosomalMarkerLAMP1(taggedwithRFP)wasco-expressedwiththewild-typeormutantAMPKβ2.Upperpanelsshowmergedimagesstainedblue(4′,6-diamidino-2-phenylindole(DAPI),nuclei),red(LAMP1,lysosomes)andgreen(AMPKβ2,detectedusingantibodyvalidatedine),incellsincubatedwithorwithoutglucosefor20 min.Lowersmallpanelsaremagnificationsoftheareasindicatedbydashedboxesintheupperpanels,showing(LtoR)redandgreenchannelsandmergedimages.
下面的这段话是图注,图注的意思我明白,但是我想知道merge后的图看什么颜色的荧光,蓝色是细胞核,红色是lysosome(位于胞质),绿色是AMPKβ2,该实验是想观察AMPKβ2是否转位到lysosome上了,如果确实发生了AMPKβ2转位到lysosome上,那么merge后是红色与绿色融合在一起,是吗?融合在一起发什么颜色的光了?

