

This product is freeze dried. All water molecules have been removed.

Every lot is tried & tested in a relevant biological assay.
Our Bioassay
-
- Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- Lazareno, S. et al. (1990) Br. J. Pharmacol. 109, 1120.
- Hernandez, M. et al. (1993) Br. J. Pharmacol. 110, 1413.
- Alomone Labs Tropicamide inhibits carbachol’s effect on M1 mAChR expressed in C6 cells.Cells were loaded with Fluo-3 AM and changes in intracellular Ca2+ were detected via changes in Fluo-3 emission. A. Normalized fluorescence before and after application (at 20 seconds, see arrow) of 10 μM Carbachol following a 20 min incubation with 0-100 μM Tropicamide (#T-120) (as indicated). B. Inhibition (as percent of control) of 10 µM Carbachol evoked Ca2+ rise, plotted against Tropicamide concentrations.
- 1. Bonner, T.I. et al. (1989) Trends. Neurosci. 12, 148.
- 2. Felder, C.C. et al. (1995) FASEB. J. 9, 619.
- 3. Bonner, T.I. et al. (1987) Science 237, 527.
- 4. Peralta, E.G. et al. (1987) EMBO J. 6, 3923.
- 5. Bonner, T.I. et al. (1988) Neuron 1, 403.
- 6. Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- 7. Liao, C.F. et al. (1989) J. Biol. Chem. 264, 7328.
- 8. Lazareno, S. et al. (1990) Br. J. Pharmacol. 109, 1120.
- 9. Hernandez, M. et al. (1993) Br. J. Pharmacol. 110, 1413.
- 10. Manny, R.E. et al. (2001) Invest. Ophtalmol. Vis. Sci. 42, 1728.
-
Muscarinic acetylcholine receptors (mAChR) regulate a number of important basic physiologic functions including heart rate, motor and sensory control and more complex behaviors including arousal, memory, and learning. Loss of muscarinic receptor number or function has been implicated in the etiology of several neurological disorders including Alzheimer"s dementia, Down"s syndrome and Parkinson"s disease.
Five subtypes of muscarinic receptors (m1-m5) have been identified by molecular cloning1 and much has been learned about their distribution, pharmacology, and structure2-6.
Tropicamide is a muscarinic receptor antagonist, which shows a 3 fold selectivity for m47-8. Its effect has a pIC50 of ~7.5 for porcine intravesical ureter contraction9.
Tropicamide produces short acting mydriasis (dilation of the pupil) and cycloplegia (paralysis of the ciliary muscle) when applied as eye drops. It is also an effective cycloplegic agent in myopic children10.
Tropicamide (#T-120) is a highly pure, synthetic, and biologically active compound.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
二、补体(complement,C)是存在于正常人和动物血清与组织液中的一组经活化后具有酶活性的蛋白质。早在19世纪末Bordet即证实,新鲜血液中含有一种不耐热的成分,可辅助和补充特异性抗体,介导免疫溶菌、溶血作用,故称为补体。补体是由30余种可溶性蛋白、膜结合性蛋白和补体受体组成的多分子系统,故称为补体系统(complementsystem)。根据补体系统各成分的生物学功能,可将其分为补体固有成分、补体调控成分和补体受体(CR)。
2 一抗是属于哪个类或亚类
二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白,因此相应的二抗就是抗IgG抗体.
单克隆抗体的类别及亚类通常会在产品列表中列出,如果你的一抗是小鼠IgM,那么相应的二抗就应当是抗小鼠IgM,或是抗小鼠IgG抗体。
如果单克隆一抗是小鼠IgG的某一亚类(IgG1,IgG2a,IgG2b,IgG3),那么几乎所有的抗小鼠IgG都可以与之结合,或者你也可以选择专门针对这一亚类的二抗,例如,如果你的一抗是小鼠IgG1,那么你可以选择抗IgG1的二抗,此种抗体在双标记实验中尤其适合。
如果你不知道一抗是哪一类别或亚类,那么抗小鼠IgG是一个不错的选择,因为此种抗体可以识别大多数类型的IgG免疫球蛋白。

