| 货号： | R30050 |
| 保存条件： | 低温 |
| 英文名： | PicoPLEXWholeGenomeAmpificationkit |
| 规格： | 50反应 |
PicoPLEX™WGAKit
WholeGenomeAmplificationforLibraryConstructionofSingleCells
“SurePlex™[PicoPLEX] representsamajorstepforwardinamplificationtechnology…weselectedSurePLEXduetoitsrapidprotocol,highlyrepresentativeamplification,andlowalleledropoutwhencomparedtootheramplificationmethods.”
–NickHaan,founderandCEOofBlueGnome,anIlluminaCompany
Overview
TheabilitytoamplifyDNAtoyieldahighlyreproduciblelibraryfroma singlecell isnowpossible!ThePicoPLEX™WGAkit withpatentedtechnology*isdesignedandoptimizedforamplificationofsinglecopygenomicDNA startingwithasinglecell. Theeasy-to-usesingletubeprotocolreduceshandlingerrors,dramaticallyimprovestimetoresultsandreducesbackground.PicoPLEXWGAKitcanbealsobeusedwithisolatedgDNAamountsranging fromlessthan15pgto50ng.AcceptedforuseinarrayCGHanalysisaswellasq-PCR.
*ProtectedbyUSPatent8,206,913andEUpendingapplications
PicoPLEX™WGAKitsareusedandtrustedbyleadingprovidersintheIVFcommunitysuchasBlueGnomeforpre-implantationgeneticscreeninganddiagnostics(PGS/PGD).
- Reproducibleresultsfromsinglecells(or<15pgDNA)
- Superiorreproducibilityofallelerepresentation
- Easytouseandautomate
- Unambiguousresultsatallresolutions,microarrayandPCR
Workflow
Learnmoreabout PicoPLEXtechnology.
- Performance
- Specifications
- Resources
- FAQs
- OrderInformation
Storeat-20°C.
Guaranteedfor12monthsat-20°Cinaconstanttemperaturefreezer.
RecommendedDNAinputs:
- 1–10humancells(e.g.blastomeres,polarbodies,trophoblasticcells,amniocytes,CTCs,culturedcells
- 1,000–10,000bacterialcells
- Intactorfragmented,single-ordouble-strandedDNA
MaximumSampleVolume,5µL
Foracompletelistofallpublications,useour ResourceSearch.
PicoPLEXWGAKitproductsheet
PicoPLEXWGA Kitmanual
MSDSPicoPLEXWGA Kit
Top10FAQs
ReadallFAQ’sonPicoPLEXWGAKit
1.WhatapplicationsarerecommendedforPicoPLEX™WGAKit?
Thiskitisusedfor:
- Copynumbervariation(CNV)analysis:superiorforoligonucleotideandBACaCGH
- SNPgenotyping
- MutationdetectionbyPCR
2.Isthereanon-stickwashbufferthatcanbeusedforsinglecellwashpriortousingPicoPLEXWGAKit?
ThishasnotbeenvalidatedatRubiconGenomics,butsomecustomershavesuccessfullyusedPVA(PolyvinylAlcohol;e.g.0.1%)inPBStowash/collecttheircells.Additionally,yourwashing/collectingbuffer(PBS)mustbealsoCa-andMg-free.
3.Aretherespecialrequirementsforflowsorting?
Yes,westronglyrecommendnotfixing,andusinglightscatteringorphasecontrasttosortorcollectsamples.Microscopic/visualconfirmationofsuccessfullysortedcellscanbeusedtooptimizesortingconditions.
4.WhatisthesampleinputvolumeforPicoPLEXWGAKit?
Thiskitwillaccommodatea5μlsampleinputvolumeperreaction(withamaximumof2.5μlofcell&buffercarryover).
5.CantheproductofPicoPLEXWGAKitbetakenstraighttoNGS?
No.
6.DoesRubiconGenomicshaveanNGS-readykitforsinglecellapplications?
Yes, PicoPLEXDNA-seq canbeusedwithIlluminaNGSplatforms.
7.Sigma-AldrichsellsaGenomePlexWGAkit,isitthesametechnologyasPicoPLEXWGA?
No,PicoPLEXusesdifferenttechnology.
8.WhatistheexpectedgenomiclocusrepresentationandLossofHeterozygosity(LOH)forPicoPLEXWGAproducts?
Forreferencepurposes,seethedataobtainedfromathirdpartyinthetablebelow.
SNPGenotypingMethod | Single-CellAmplificationSuccessRate | SNPCallRates | LOH |
PCR | 95% | >95% | <10% |
IlluminaInfiniumSNParray | 95% | 50%–60% | 7%–12% |
9.Istheproductofthistechnologysingle-ordouble-stranded?
TheendproductofPicoPLEXWGAisamixtureofsingle-anddouble-strandedDNA.
10.HowrapidisPicoPLEXWGAKit?
PicoPLEXWGAKitlysescellsandamplifiestheDNAtoanend-pointof2–5μgin<3hours.
PicoPLEX™WGAKit,50reactions CAT.NO.R30050
