中国SBI公司 SBI(systembio)代理商,SBI/pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)/CYTO120R-PA-1/10 µg,RNA干扰,SBI,System Biosciences（SBI）,,10 µg蚂蚁淘商城

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SBI/pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)/CYTO120R-PA-1/10 µg

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SBI/pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)/CYTO120R-PA-1/10 µg

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systembio

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CYTO120R-PA-1

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Overview:

EasilytrackexosomeswithaCD63-RFPfusion

MonitorexosomedynamicswiththepCT-CD63-RFP(pCMV)ExosomeCyto-Tracer(alsoavailableasaCD63-GFPfusion,pCT-CD63-GFP).Providedaseitherlentivectororpre-packagedvirus,thisCyto-TracerfusesRFPtothetetraspaninCD63,whichthengetspackagedintoexosomesproducedbythetransfectedcells.GreatforcreatingstablecelllinesthatproduceRFP-labeledexosomesaswellaslabelingexosomesfromprimarycells,tumorcells,stemcells,andmore.

Stablelentivector-basedsystem
Idealforco-localizationstudies
Monitorexosomedynamicsandfunctionalstudiesinrealtime
Labelexosomesfromprimarycells,tumorcells,stemcells,andmore

Choosetheexosomequantitationmethodthat’sbestforyourstudies

Promoter(Cat.#)	Cyto-Tracer	TargetLocation	PeptideTag
CMV(CYTO100-PA-1/CYTO100-VA-1) MSCV(CYTO100-PB-1/CYTO100-VB-1)	pCT-Mem-GFP	PlasmaMembrane	Neuromodulin
CMV(CYTO101-PA-1/CYTO101-VA-1) MSCV(CYTO101-PB-1/CYTO101-VB-1)	pCT-InnerMem-GFP	InnerLeafletofMembrane	Farnesylationsignal
CMV(CYTO102-PA-1/CYTO102-VA-1) MSCV(CYTO102-PB-1/CYTO102-VB-1)	pCT-Mito-GFP	Mitochondria	CytochromeCoxidaseVIII
CMV(CYTO103-PA-1/CYTO103-VA-1)	pCT-ER-GFP	EndoplasmicReticulum	ERtargetingconsensus
CMV(CYTO104-PA-1/CYTO104-VA-1)	pCT-Golgi-GFP	GolgiApparatus	Galactosyltrasferase
CMV(CYTO105-PA-1/CYTO105-VA-1)	pCT-Secretory-GFP	ER-Golgi Vesicles	Secretoryconsensuspeptide
CMV(CYTO106-PA-1/CYTO106-VA-1)	pCT-Lyso-GFP	Lysosome	CathepsinB
CMV(CYTO107-PA-1/CYTO107-VA-1)	pCT-Endo-GFP	Endosome	RhoB
CMV(CYTO107R-PA-1/CYTO107R-VA-1)	pCT-Endo-RFP	Endosome	RhoB
CMV(CYTO108-PA-1/CYTO108-VA-1) MSCV(CYTO108-PB-1/CYTO108-VB-1)	pCT-Pero-GFP	Peroxisome	Peroxisomalconsensussignal
CMV(CYTO120-PA-1/CYTO120-VA-1)	pCT-Autophago-GFP	Autophagosome	LC3
CMV(CYTO120R-PA-1/CYTO120R-VA-1)	pCT-Autophago-RFP	Autophagosome	LC3
CMV(CYTO109-PA-1/CYTO109-VA-1)	pCT-CD63-GFP	Exosome/Secretory	CD63Tetraspanin
CMV(CYTO109R-PA-1/CYTO109R-VA-1)	pCT-CD63-RFP	Exosome/Secretory	CD63Tetraspanin
CMV(CYTO122-PA-1/CYTO122-VA-1)	pCT-CD9-GFP	Exosome/Secretory	CD9Tetraspanin
CMV(CYTO123-PA-1/CYTO123-VA-1)	pCT-CD9-RFP	Exosome/Secretory	CD9Tetraspanin
CMV(CYTO124-PA-1/CYTO124-VA-1)	pCT-CD81-GFP	Exosome/Secretory	CD81Tetraspanin
CMV(CYTO125-PA-1/CYTO125-VA-1)	pCT-CD81-RFP	Exosome/Secretory	CD81Tetraspanin
CMV(CYTO110-PA-1/CYTO110-VA-1) MSCV(CYTO110-PB-1/CYTO110-VB-1)	pCT-Actin-GFP	Cytoskeleton	β-actin
CMV(CYTO111-PA-1/CYTO111-VA-1) MSCV(CYTO111-PB-1/CYTO111-VB-1)	pCT-Tublin-GFP	Cytoskeleton	α-tublin
CMV(CYTO111R-PA-1/CYTO111R-VA-1)	pCT-Tublin-RFP	Cytoskeleton	α-tublin
CMV(CYTO112-PA-1/CYTO112-VA-1)	pCT-MAP4-GFP	Microtubule	Microtubuleassociatedprotein4(MAP4)
CMV(CYTO113-PA-1/CYTO113-VA-1) MSCV(CYTO113-PB-1/CYTO113-VB-1)	pCT-H2B-GFP	Nucleus	H2B
CMV(CYTO114-PA-1/CYTO114-VA-1)	pCT-Apoptosis-Luc	Caspase3/7activation	Cyclicinactiveluciferase
CMV(CYTO115-PA-1/CYTO115-VA-1)	pCT-GFP-BAX	CytosoltoMitochondria	BAX
CMV(CYTO116-PA-1/CYTO116-VA-1)	pCT-Catenin-GFP	CytosoltoNucleus/CellMembrane	β-Cateninactivation
CMV(CYTO117-PA-1/CYTO117-VA-1)	pCT-CMV-PSD95-GFP	DendriteMembranes	PSD-95
CMV(CYTO118-PA-1/CYTO118-VA-1) MSCV(CYTO118-PB-1/CYTO118-VB-1)	pCT-Cyto-GFP	CytosolicCompartment	GFP-Untagged
CMV(CYTO119-PA-1/CYTO119-VA-1)	pCT-Cyto-RFP	CytosolicCompartment	RFP-Untagged
MSCV(CYTO121-PB-1/CYTO121-VB-1)	pCT-Cyto-GFpLuc	GFPandLuciferaseControl	GFPandLuciferase(Untagged)

SupportingData:

SeesomeofourexosomeCyto-Tracersinaction

Thefollowingfigureandvideosarefrom:

GarciaNA,etal.GlucoseStarvationinCardiomyocytesEnhancesExosomeSecretionandPromotesAngiogenesisinEndothelialCells.PLoSONE.2015.10(9).PMCID:PMC4578916.

See exosomes being transferred from cardiomyocytes to mouse endothelial cells and human cord blood endothelial cells (HUVEC)

Figure4fromGarcia,etal.ExosometransferfromCMstoECs.(A)H9c2transfectedwithpCT-CD63-GFP.FACSanalysisof90%GFPpositivecells.(B)Representativeimagesfromconfocaltime-lapsemicroscopyofmouseECs(ACTB-DsRed)(EC;red)co-culturedwithH9C2-CD63-GFPcells(green),previouslyculturedfor24hin+/-Stmedium.ExosometransferfromH9C2CMstoECcanbeobserved(S1andS2Movies,below).WhitearrowsshowCD63-GFPstructuresinsideECs(C)RepresentativeimmunostainingofH9C2-CD63-GFPandHUVECco-cultures;anti-GFP(green)andanti-CD31(red).TheimagesillustrateGFPfluorescencefromCD63-GFPexosomesinCD31-positivecells(red)after24hincubationin+/-Stmedium.WhitearrowsshowCD63-GFPstructuresinsideECs.