![Immunoway/MCM3 (phospho-Ser112) rabbit pAb/20ug 50ug 100ug/YP1398](images/no_picture.gif)
MCM3 (phospho-Ser112)rabbit pAb
- Catalog No.:YP1398
- Applications:WB
- Reactivity:Human
- Data Sheet
- MSDS
- Support
- Description
- References ( 0 )
- Protocol
- Gene Name:
- MCM3
- Protein Name:
- MCM3 (Ser112)
- Human Gene Id:
- 4172
- Human Swiss Prot No:
- P25205
- Mouse Gene Id:
- 17215
- Mouse Swiss Prot No:
- P25206
- Immunogen:
- Synthesized phosho peptide around human MCM3 (Ser112)
- Specificity:
- This antibody detects endogenous levels ofHuman MCM3 (phospho-Ser112)
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Rabbit
- Dilution:
- WB 1:1000-2000
- Purification:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -20°C/1 year
- Other Name:
- DNA replication licensing factor MCM3 (EC 3.6.4.12) (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3) (RLF subunit beta) (p102)
- Observed Band(KD):
- 89
- Background:
- minichromosome maintenance complex component 3(MCM3)Homo sapiensThe protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012],
- Function:
- function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.,miscellaneous:Associated with the replication-specific DNA polymerase alpha.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,
- Subcellular Location:
- nuclear chromosome, telomeric region,nucleus,nucleoplasm,alpha DNA polymerase:primase complex,centrosome,membrane,MCM complex,intracellular membrane-bounded organelle,perinuclear region of cytoplasm,
- Expression:
- Cervix,Coronary artery,Epithelium,Lymph,Osteosarcoma,Pancre
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- June 19-2018
- FLOW CYTOMEYRT PROTOCOL
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN
- July 13-2018
- Antibody-FAQs
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研究蛋白质芯片的意义
1。蛋白质是基因表达的最终产物,接近生命活动的物质层面;
2。探针蛋白特异性高、亲和力强,可简化样品前处理,甚至可直接利用生物材料(血样、尿样、细胞及组织等)进行检测;
3。适合高通量筛选与靶蛋白作用的化合物;
4。有助于了解药物或毒物与其效应相关蛋白质的相互作用。
蛋白质芯片的分类:
1.蛋白质检测芯片
2.蛋白质功能芯片
蛋白质芯片的制备:
1。固相载体及其处理
载体(滴定板、滤膜、凝胶、载玻片)
2。蛋白质的预处理
选择具有较高纯度和完好生物活性的蛋白进行溶解
3。点制微阵列
可使用点制基因微阵列的商品化点样仪或喷墨法等
4。膜为载体:芯片放入湿盒,37°C1h
载玻片为载体:化学修饰产生醛基固定蛋白
5。微阵列的封闭固定微阵列上的蛋白样点
主要封闭试剂:BSA或Gly
相关链接:
全部有关生物芯片的实验方法技术(protocol)
生物芯片相关仪器及芯片・芯片扫描仪・芯片点样仪・生物芯片・生物芯片系统・其它
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核酸分析类试剂
AFLP分析|SNP基因分型|线粒体DNA基因分型|其它基因分型|DNA指纹试剂盒|DNA测序试剂|核酸电泳凝胶|核酸标准品|凝胶纯化试剂盒|核酸染色|转座工具|其它
求助各位大神,点击Downloadfulltable无法下载,总是出现错误,已经排除网络的问题,不知道是否还有其他方法下载,上面有一个Datatable的表格,不知道是不是芯片平台文件?
请问,我通过基因芯片筛选差异基因,有上调的也有下调的,即FC有正值,亦有负值,那么负值怎么取log?谢谢
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做蛋白质和microRNA的关系,那么先找出差异的蛋白质还是找出差异的microRNA好呢?有没有做ITRAQ和MICRORNA芯片好的公司推荐,非常感谢!
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1、一张芯片上可以同时分析成百上千的探针,这样的情况可以检测多少位点?
2、基因芯片技术通量会有多大?怎么计算?
从GEO和ARRAYEXPRESS下载了miRNA的表达数据矩阵,但是结果不太一样,大致两种:①数值从个位数到几万不等,而且没有小数点;②数值在10上下,有小数点。见图
请问怎么知道数据是否经过了log转换?有注释文件说明吗?还是直接判断①是没转换的,②是转换的?
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