Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 105553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV1 | 105553-AAV1 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV5 | 105553-AAV5 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV9 | 105553-AAV9 | Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information | Add to Cart | |||
| AAV Retrograde | 105553-AAVrg | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberUnknown
Gene/Insert
- Gene/Insert nameCre
- PromoterhSyn
Cloning Information
- Cloning methodUnknown
Resource Information
- Supplemental Documents
- pENN.AAV.hSyn.Cre.WPRE.hGH (p2676).gb
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
Penn Vector Core number p2676
Information for AAV1 (Catalog # 105553-AAV1)(Back to top)
Purpose
Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA.
hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV1
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Data submitted about 105553-AAV1 by requesting scientist(s):
- Data 1: Mouse, Brain parenchyma
Information for AAV5 (Catalog # 105553-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA.
hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV9 (Catalog # 105553-AAV9)(Back to top)
Purpose
Ready-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA.
hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 1×10¹³ vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap gene
- BufferPBS + 0.001% Pluronic F-68
- SerotypeAAV9
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Ancillary Agreement for Penn Vectors
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Information for AAV Retrograde (Catalog # 105553-AAVrg)(Back to top)
Purpose
Ready-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA.
hSyn-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV retrograde (AAVrg)
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Data submitted about 105553-AAVrg by requesting scientist(s):
- Data 1: Mouse, Brain parenchyma
- Data 2: Rat, Brain parenchyma
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求助各位大神，点击Downloadfulltable无法下载，总是出现错误，已经排除网络的问题，不知道是否还有其他方法下载，上面有一个Datatable的表格，不知道是不是芯片平台文件？
请问，我通过基因芯片筛选差异基因，有上调的也有下调的，即FC有正值，亦有负值，那么负值怎么取log？谢谢
中唐临界风险，33岁，第一胎，然后羊水穿刺加CMA基因芯片检查，染色体核型正常，4维彩超正常，医生说发育速度偏慢，但还算正常范围，CMA结果异常，如照片
从GEO和ARRAYEXPRESS下载了miRNA的表达数据矩阵，但是结果不太一样，大致两种：①数值从个位数到几万不等，而且没有小数点；②数值在10上下，有小数点。见图
请问怎么知道数据是否经过了log转换？有注释文件说明吗？还是直接判断①是没转换的，②是转换的？
做蛋白质和microRNA的关系，那么先找出差异的蛋白质还是找出差异的microRNA好呢？有没有做ITRAQ和MICRORNA芯片好的公司推荐，非常感谢！
无创dna检测8号染色体缺失32.99mb，羊穿正常，基因芯片检测还是8号染色体杂合性缺失32.2mb，请教专家孩子能要吗？万分感谢
1、一张芯片上可以同时分析成百上千的探针，这样的情况可以检测多少位点？
2、基因芯片技术通量会有多大？怎么计算？
研究蛋白质芯片的意义
1。蛋白质是基因表达的最终产物,接近生命活动的物质层面；
2。探针蛋白特异性高、亲和力强,可简化样品前处理，甚至可直接利用生物材料(血样、尿样、细胞及组织等)进行检测；
3。适合高通量筛选与靶蛋白作用的化合物；
4。有助于了解药物或毒物与其效应相关蛋白质的相互作用。
蛋白质芯片的分类：
1.蛋白质检测芯片
2.蛋白质功能芯片
蛋白质芯片的制备：
1。固相载体及其处理
载体（滴定板、滤膜、凝胶、载玻片）
2。蛋白质的预处理
选择具有较高纯度和完好生物活性的蛋白进行溶解
3。点制微阵列
可使用点制基因微阵列的商品化点样仪或喷墨法等
4。膜为载体：芯片放入湿盒，37°C1h
载玻片为载体：化学修饰产生醛基固定蛋白
5。微阵列的封闭固定微阵列上的蛋白样点
主要封闭试剂：BSA或Gly
相关链接:
全部有关生物芯片的实验方法技术(protocol)
生物芯片相关仪器及芯片・芯片扫描仪・芯片点样仪・生物芯片・生物芯片系统・其它
生物芯片技术服务
核酸分析类试剂
AFLP分析|SNP基因分型|线粒体DNA基因分型|其它基因分型|DNA指纹试剂盒|DNA测序试剂|核酸电泳凝胶|核酸标准品|凝胶纯化试剂盒|核酸染色|转座工具|其它
