DescriptionViPrimePLUS One Step Taq RT-qPCR Master Mix is next generation master mix designed for one step realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step Taq RT-qPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.ViPrimePLUS One Step Taq RT-qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RTqPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Master Mix is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.
Features
- One step real time RT-qPCR reaction set up
- Equipped with thermostable M-MULV enzyme
- Good buffer system for excellent amplification efficiency
- High sensitivity detection
- Optimal performance for highly sensitive and specific RT-qPCR reaction
- Compatible with most of the real-time PCR platforms
Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step Taq RTqPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM13ViPrimePLUS One Step Taq RT-qPCR Master Mix | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM13-LRViPrimePLUS One Step Taq RT-qPCR Master Mix with Low ROX | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM13-RViPrimePLUS One Step Taq RT-qPCR Master Mix with ROX | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM13 | ViPrimePLUS One Step Taq RT-qPCR MasterMix | 150 reactions |
| QLMM13-LR | ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX | 150 reactions |
| QLMM13-R | ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX | 150 reactions |
DownloadManual
ViPrimePLUS One Step Taq RT-qPCR MasterMix ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX
PublicationThis Product Has Been Used In:Attaran, B., Falsafi, T. (2017).). Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics, . World Journal of Gastroenterology, Vol. 23, No. 7. 1163-1170 (2017).
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研究蛋白质芯片的意义
1。蛋白质是基因表达的最终产物,接近生命活动的物质层面；
2。探针蛋白特异性高、亲和力强,可简化样品前处理，甚至可直接利用生物材料(血样、尿样、细胞及组织等)进行检测；
3。适合高通量筛选与靶蛋白作用的化合物；
4。有助于了解药物或毒物与其效应相关蛋白质的相互作用。
蛋白质芯片的分类：
1.蛋白质检测芯片
2.蛋白质功能芯片
蛋白质芯片的制备：
1。固相载体及其处理
载体（滴定板、滤膜、凝胶、载玻片）
2。蛋白质的预处理
选择具有较高纯度和完好生物活性的蛋白进行溶解
3。点制微阵列
可使用点制基因微阵列的商品化点样仪或喷墨法等
4。膜为载体：芯片放入湿盒，37°C1h
载玻片为载体：化学修饰产生醛基固定蛋白
5。微阵列的封闭固定微阵列上的蛋白样点
主要封闭试剂：BSA或Gly
相关链接:
全部有关生物芯片的实验方法技术(protocol)
生物芯片相关仪器及芯片・芯片扫描仪・芯片点样仪・生物芯片・生物芯片系统・其它
生物芯片技术服务
核酸分析类试剂
AFLP分析|SNP基因分型|线粒体DNA基因分型|其它基因分型|DNA指纹试剂盒|DNA测序试剂|核酸电泳凝胶|核酸标准品|凝胶纯化试剂盒|核酸染色|转座工具|其它
1、一张芯片上可以同时分析成百上千的探针，这样的情况可以检测多少位点？
2、基因芯片技术通量会有多大？怎么计算？
请问，我通过基因芯片筛选差异基因，有上调的也有下调的，即FC有正值，亦有负值，那么负值怎么取log？谢谢
做蛋白质和microRNA的关系，那么先找出差异的蛋白质还是找出差异的microRNA好呢？有没有做ITRAQ和MICRORNA芯片好的公司推荐，非常感谢！
无创dna检测8号染色体缺失32.99mb，羊穿正常，基因芯片检测还是8号染色体杂合性缺失32.2mb，请教专家孩子能要吗？万分感谢
求助各位大神，点击Downloadfulltable无法下载，总是出现错误，已经排除网络的问题，不知道是否还有其他方法下载，上面有一个Datatable的表格，不知道是不是芯片平台文件？
中唐临界风险，33岁，第一胎，然后羊水穿刺加CMA基因芯片检查，染色体核型正常，4维彩超正常，医生说发育速度偏慢，但还算正常范围，CMA结果异常，如照片
从GEO和ARRAYEXPRESS下载了miRNA的表达数据矩阵，但是结果不太一样，大致两种：①数值从个位数到几万不等，而且没有小数点；②数值在10上下，有小数点。见图
请问怎么知道数据是否经过了log转换？有注释文件说明吗？还是直接判断①是没转换的，②是转换的？
