
Biocare’sPromARK™seriesintroducesRabbit-on-CanineHRP-PolymerDetection.Thisdetectionisspeciallydesignedforusingrabbitantibodiesoncanineandfelinetissuesandutilizesbiotin-freehorserADIshperoxidase(HRP)polymertechnology.Theadvantagesofthispolymertechnologyareincreasedsensitivity,reductionofIHCsteps(noAvidin/BiotinblockorLink/Probe),andminimalcross-reactivitytoendogenouscanineandfelineIgG.TheRabbit-on-Caninedetectioncanbeusedwithparaffin-embeddedtissuesandcanbeperformedmanuallyoronautomatedstainingplatforms.TheadditionofproprietaryblockerstothedetectionreagentpermitstheuseofanyofBiocareMedical’sretrievalsolutions(Reveal/Borg/Diva)orenzymeswiththeeliminationofendogenousIgGornon-specificbackgroundstaining.TemperaturedependentprotocolscanbeconvenientlyperformedusingBiocare’sDecloakingChamber.TheRabbit-on-CanineHRP-PolymermaybecombinedwiththeMouse-on-CanineHRP-PolymertocreateauniversalpolymerdetectionthatwilllabelbothmouseandrabbitantibodieswithHRP.RefertoTechnicalNote#4foradditionalinformation.TheRabbit-on-CanineHRP-PolymermaybecombinedwiththeMouse-on-CanineAP-PolymertocreateadoublestainpolymerdetectionthatwilllabeltherabbitantibodywithHRPandthemouseantibodywithAP.RefertoTechnicalNotes#5and#6foradditionalinformation.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
设计方法:通用茎环后端加miRNA后面6个碱基的反向互补序列为反转录引物,正向引物为miRNA去除后面6个碱基的序列,反向引物在茎环上。
反转录茎环通用序列:
GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGAC
miRNA序列:
>mmu-miR-99b-5p
MIMAT0000132 Mus musculus miR-99b-5p
CACCCGUAGAACCGACCUUGCG
mmu-miR-99b-5p反转录颈环引物为:
GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCGCAAG
定量PCR反向引物为:TGTCGTGGAGTCGGC
正向引物为:CACCCGTAGAACCGAC
http://medgen.ugent.be/rtprimerdb/

