Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE.CBBG can also be used with the Bradford Method to determine protein concentration.G-250 is differentiated from the R-250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color.CBBG may exist in one of three forms: a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm.The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex—forming the basis of the Bradford Method.
Recently, it has also been shown that CBBG is also an antagonist to P2X7 (P2X purinoceptor 7: P2RX7).CBBG is soluble in water but methanol and acetic acid are often added for better solubility.
Product SpecificationsComassie Brilliant Blue G-250, Ultra PureCBBG, Ultra Pure
Formula: C47H48N3O7S2Na
MW: 854.02 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 61363
GoldBio活体成像技术：早在1999年由美国哈佛大学Weissleder博士率先提出了分子影像学(molecularimaging，MI)的概念，即应用影像学的方法对活体状态下的生物过程进行细胞和分子水平的定性和定量研究。活体成像便是基于分子影像学孕育而生的，通过这个成像系统，可以观测活体动物体内肿瘤的生长及转移，感染性疾病的发展进程，特定基因的表达等生物学过程。活体成像技术主要采用生物发光（bioluminescence）与荧光（fluorescence）两种技术。★生物发光是用荧光素酶基因标记细胞或DNA。★荧光技术则采用荧光报告基团（GFP、RFP,Cyt及dyes等）进行标记。★这一技术对肿瘤微小转移灶的检测灵敏度极高，不涉及放射性物质和方法,非常安全。操作极其简单、所得结果直观、灵敏度高。
活体成像两种检测技术介绍活体成像特点优点缺点生物发光检测bioluminescence★荧光素酶（Luciferase）对基因、细胞和活体动物进行标记；★荧光素酶催化底物（例如荧光素钾盐）反应后，会产生化学发光。这种光是由化学反应而来，不需要激发光；★标记方法是通过克隆技术，将荧光素酶的基因插入到预期观察的细胞染色体内，通过对克隆细胞进行筛选，培养出能稳定表达荧光素酶的细胞株。再将细胞株转移至特定的小鼠体内形成模型。★特异性强，无自发荧光；★高灵敏度，在体内可检测到几百个细胞，检测的深度在3-100px；★定量精确 ★信号较弱，检测时间较长；★仪器精密度要求较高；★细胞或基因需要转基因标记；★不可用于人体，不适用于抗体、多肽等标记荧光检测fluorescence★采用荧光报告基因(GFP、RFP等)或荧光染料进行标记；★需要外接激发光源，利用报告基因、荧光蛋白质或染料产生的荧光，就可以形成体内的生物光源。★荧光染料、蛋白标记能力强；★信号强，成像速度快，操作简便，实验成本较低；★未来可用于人；★适用范围广，可以是动物、细胞、微生物，也可以是抗体、药物、纳米材料等。★存在自发荧光，影响灵敏度；★光容易被动物组织吸收；★检测深度受限；★背景光干扰，定量准确度低
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
