PepMix™HAdV-3(hexonprotein)
ProductCode:PM-HAdV3
Poolof234peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughHexonprotein(Swiss-ProtID:P36849)ofHumanadenovirus3(HAdV-3)forTcellassays(e.g.ELISPOT).
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Infection,Respiratoryinfection
DeliveryTime:2-5days
PepMix™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
PepMix™HAdV-3(hexonprotein)
SelectedReferences
"InVitroStudiesoftheImpactofMaribavironCMV-SpecificCellularImmuneResponses"
Stacheletal.,JClinVirol. (2015)-PMID:26780109
"InductionOfBroad-basedImmunityandProtectiveEfficacybySelf-amplifyingmRNAVaccinesEncodingInfluenzaVirusHemagglutinin"
Brazzolietal.,JournalofVirology (2015)-PMID:26468547
"TargetingofNucleoproteintoChemokineReceptorsbyDNAVaccinationResultsinIncreasedCD8+-mediatedCrossProtectionAgainstInfluenza"
Baranowskaetal.,Vaccine(2015)-PMID:26387432
"CharacteristicsofImmuneMemory10-15yearsAfterPrimaryHepatitisBVaccination"
Hummeletal.,Vaccine(2015)-PMID:n.a.
"TCellsSpecificforDifferentLatentandLyticViralProteinsEfficientlyControlEpstein-BarrVirusTransformedBCells"
Nowakowskaetal.,Cytotherapy(2015)-PMID:26276009
"High-densityPrecultureofPBMCsRestoresDefectiveSensitivityofCirculatingCD8TCellstoVirus-andTumor-derivedAntigens"
Wegneretal.,Blood(2015)-PMID:26024876
"MetabolicRegulationofHepatitisBImmunopathologybyMyeloid-derivedSuppressorCells"
Pallettetal.,NatureMedicine(2015)-PMID:25962123
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK
PepMix™HAdV-3(hexonprotein)
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Hexonprotein |
| Organism | Humanadenovirus |
| Indication/Topic | Respiratoryinfection Respiratoryinfection |
| Numberofpeptides | 234 |
FurtherInformation:
| Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | ProtocolandDatasheet |
| Swiss-ProtID | P36849 |
| NCBI | YP_002213779 |
| Sequence | MATPSMMPQWAYMHIAGQDASGYLSPGL VQFARATDTYFSMGNKFRNPTVAPTHDV TTDRSQRLMLRFVPVDREDNTYSYKVRY TLAVGDNRVLDMASTFFDIRGVLDRGPS FKPYSGTAYNSLAPKGAPNTSQWIVTTN GDNAVTTTTNTFGIASMKGGNITKEGLQ IGKDITTTEGEEKPIYADKTYQPEPQVG EESWTDTDGTNEKFGGRALKPATNMKPC YGSFARPTNIKGGQAKNRKVKPTTEGGV ETEEPDIDMEFFDGRDAVAGALAPEIVL YTENVNLETPDSHVVYKPETSNNSHANL GQQAMPNRPNYIGFRDNFVGLMYYNSTG NMGVLAGQASQLNAVVDLQDRNTELSYQ LLLDSLGDRTRYFSMWNQAVDSYDPDVR IIENHGIEDELPNYCFPLNGIGPGHTYQ GIKVKTDDTNGWEKDANVAPANEITIGN NLAMEINIQANLWRSFLYSNVALYLPDV YKYTPPNITLPTNTNTYEYMNGRVVSPS LVDSYINIGARWSLDPMDNVNPFNHHRN AGLRYRSMLLGNGRYVPFHIQVPQKFFA VKNLLLLPGSYTYEWNFRKDVNMVLQSS LGNDLRTDGATISFTSINLYATFFPMAH NTASTLEAMLRNDTNDQSFNDYLSAANM LYPIPANATNIPISIPSRNWAAFRGWSF TRLKTKETPSLGSGFDPYFVYSGSIPYL DGTFYLNHTFKKVAIMFDSSVSWPGNDR LLSPNEFEIKRTVDGEGYNVAQCNMTKD WFLVQMLANYNIGYQGFYIPEGYKDRMY SFFRNFQPMSRQVVDEVNYTDYKAVTLP YQHNNSGFVGYLAPTMRQGEPYPANYPY PLIGTTAVKSVTQKKFLCDRTMWRIPFS SNFMSMGALTDLGQNMLYANSAHALDMT FEVDPMDEPTLLYLLFEVFDVVRVHQPH RGVIEAVYLRTPFSAGNATT |
| ProteinLength(aa) | 944 |
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
