PepMix™HCMVA(UL48)_2subpools
ProductCode:PM-UL48
Poolof2x281peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughDeneddylaseUL48(Swiss-ProtID:P16785)ofHumancytomegalovirus(HHV-5)forTcellassays.
Amount:2vials(oneofeachsubpool)containing15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Infection,Cancer,TORCH,Infectiousmononucleosis,CMVhepatitis,Retinitis,Colitis,Pneumonitis,Esophagitis,AIDS,Cancerchemotherapy,Transplantation
DeliveryTime:2-5days
PepMix™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
PepMix™HCMVA(UL48)_2subpools
SelectedReferences
"InVitroStudiesoftheImpactofMaribavironCMV-SpecificCellularImmuneResponses"
Stacheletal.,JClinVirol. (2015)-PMID:26780109
"InductionOfBroad-basedImmunityandProtectiveEfficacybySelf-amplifyingmRNAVaccinesEncodingInfluenzaVirusHemagglutinin"
Brazzolietal.,JournalofVirology (2015)-PMID:26468547
"TargetingofNucleoproteintoChemokineReceptorsbyDNAVaccinationResultsinIncreasedCD8+-mediatedCrossProtectionAgainstInfluenza"
Baranowskaetal.,Vaccine(2015)-PMID:26387432
"CharacteristicsofImmuneMemory10-15yearsAfterPrimaryHepatitisBVaccination"
Hummeletal.,Vaccine(2015)-PMID:n.a.
"TCellsSpecificforDifferentLatentandLyticViralProteinsEfficientlyControlEpstein-BarrVirusTransformedBCells"
Nowakowskaetal.,Cytotherapy(2015)-PMID:26276009
"High-densityPrecultureofPBMCsRestoresDefectiveSensitivityofCirculatingCD8TCellstoVirus-andTumor-derivedAntigens"
Wegneretal.,Blood(2015)-PMID:26024876
"MetabolicRegulationofHepatitisBImmunopathologybyMyeloid-derivedSuppressorCells"
Pallettetal.,NatureMedicine(2015)-PMID:25962123
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-StimulatedExpansionofVirus-specificTcellsforPreventativeTreatmentafterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK
PepMix™HCMVA(UL48)_2subpools
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | DeneddylaseUL48 |
| Organism | Humancytomegalovirus(HHV-5) |
| Indication/Topic | Cancer Infectiousmononucleosis OpportuNISTicinfections |
| Numberofpeptides | 2x281 |
FurtherInformation:
| Amount | 2vials(oneofeachsubpool)containing15nmol(appr.25µg)perpeptide |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | ProtocolandDatasheet |
| Swiss-ProtID | P16785 |
| NCBI | CAA35407.1 |
| Sequence | MKVTQASCHQGDIARFGARAGNQCVCNG IMFLHALHLGGTSAVLQTEALDAIMEEG ARLDARLERELQKKLPAGGRLPVYRLGD EVPRRLESRFGRTVHALSRPFNGTTETC DLDGYMCPGIFDFLRYAHAKPRPTYVLV TVNSLARAVVFTEDHMLVFDPHSSAECH NAAVYHCEGLHQVLMVLTGFGVQLSPAF YYEALFLYMLDVATVPEAEIAARLVSTY RDRDIDLTGVVRESADTAATTTTAAPSL PPLPDPIVDPGCPPGVAPSIPVYDPSSS PKKTPEKRRKDLSGSKHGGKKKPPSTTS KTLATASSSPSAIAAASSSSAVPPSYSC GEGALPALGRYQQLVDEVEQELKALTLP PLPANTSAWTLHAAGTESGANAATATAP SFDEAFLTDRLQQLIIHAVNQRSCLRRP CGPQSAAQQAVRAYLGLSKKLDAFLLNW LHHGLDLQRMHDYLSHKTTKGTYSTLDR ALLEKMQVVFDPYGRQHGPALIAWVEEM LRYVESKPTNELSQRLQRFVTKRPMPVS DSFVCLRPVDFQRLTQVIEQRRRVLQRQ REEYHGVYEHLAGLITSIDIHDLDASDL NRREILKALQPLDDNAKQELFRLGNAKM LELQMDLDRLSTQLLTRVHNHILNGFLP VEDLKQMERVVEQVLRLFYDLRDLKLCD GSYEEGFVVIREQLSYLMTGTVRDNVPL LQEILQLRHAYQQATQQNEGRLTQIHDL LHVIETLVRDPGSRGSALTLALVQEQLA QLEALGGLQLPEVQQRLQNAQLALSRLY EEEEETQRFLDGLSYDDPPNEQTIKRHP QLREMLRRDEQTRLRLINAVLSMFHTLV MRLARDESPRPTFFDAVSLLLQQLPPDS HEREDLRAANATYAQMVKKLEQIEKAGT GASEKRFQALRELVYFFRNHEYFFQHMV GRLGVGPQVTELYERYQHEMEEQHLERL EREWQEEAGKLTVTSVEDVQRVLARAPS HRVMHQMQQTLTTKMQDFLDKEKRKQEE QQRQLLDGYQKKVQQDLQRVVDAVKGEM LSTIPHQPLEATLELLLGLDQRAQPLLD KFNQDLLSALQQLSKKLDGRINECLHGV LTGDVERRCHPHREAAMQTQASLNHLDQ ILGPQLLIHETQQALQHAVHQAQFIEKC QQGDPTTAITGSEFEGDFARYRSSQQKM EEQLQETRQQMTETSERLDRSLRQDPGS SSVTRVPEKPFKGQELAGRITPPPADFQ QPVFKTLLDQQADAARKALSDEADLLNQ KVQTQLRQRDEQLSTAQNLWTDLVTRHK MSGGLDVTTPDAKALMEKPLETLRELLG KATQQLPYLSAERTVRWMLAFLEEALAQ ITADPTHPHHGSRTHYRNLQQQAVESAV TLAHQIEQNAACENFIAQHQEATANGAS TPRVDMVQAVEAVWQRLEPGRVAGGAAR HQKVQELLQRLGQTLGDLELQETLATEY FALLHGIQTFSYGLDFRSQLEKIRDLRT RFAELAKRRGTRLSNEGVLPNPRKPQAT TSLGAFTRGLNALERHVQLGHQYLLNKL NGSSLVYRLEDIPSVLPATHETDPALIM RDRLRRLCFARHHDTFLEVVDVFGMRQI VTQAGEPIHLVTDYGNVAFKYLALRDDG RPLAWRRRCSGGGLKNVVTTRYKAITVA VAVCQTLRTFWPQISQYDLRPYLTQHQS HTHPAETHTLHNLKLFCYLVSTAWHQRI DTQQELTAADRVGSGEGGDVGEQRPGRG TVLRLSLQEFCVLIAALYPEYIYTVLKY PVQMSLPSLTAHLHQDVIHAVVNNTHKM PPDHLPEQVKAFCITPTQWPAMQLNKLF WENKLVQQLCQVGPQKSTPPLGKLWLYA MATLVFPQDMLQCLWLELKPQYAETYAS VSELVQTLFQIFTQQCEMVTEGYTQPQL PTGEPVLQMIRVPRQDTTTTDTNTTTEP GLLDVFIQTETALDYALGSWLFGIPVCL GVHVADLLKGQRILVARHLEYTSRDRDF LRIQRSRDLNLSQLLQDTWTETPLEHCW LQAQIRRLRDYLRFPTRLEFIPLVIYNA QDHTVVRVLRPPSTFEQDHSRLVLDEAF PTFPLYDQDDNSSADNIAASGAAPTPPV PFNRVPVNIQFLRENPPPIARVQQPPRR HRHRAAAAADDDGQIDHVQDDTSRTADS ALVSTAFGGSVFQENRLGETPLCRDELV AVAPGAASTSFASPPITVLTQNVLSALE ILRLVRLDLRQLAQSVQDTIQHMRFLYL L |
| ProteinLength(aa) | 2241 |
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
