PepMix™Human(Actin)
ProductCode:PM-ACTS
NegativeControlPoolof92peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughActin,alphaskeletalmuscle(Swiss-ProtID:P68133)ofHomosapiens(Human)forTcellassays.
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Control
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
PepMix™Human(Actin)
SelectedReferences
"StandardizationofCryopreservedPeripheralBloodMononuclearCellsThroughaRestingProcessforClinicalImmunomonitoring?DevelopmentofanAlgorithm"
Wangetal.,CytometryA.(2015)-PMID:26848928
"DichotomousEffectsofLatentCMVInfectiononthePhenotypeandFunctionalPropertiesofCD8+T-cellsandNK-cells"
Bigleyetal.,CellularImmunology(2015)-PMID:n.a
"K562-DerivedWhole-CellVaccineEnhancesAntitumorResponsesofCAR-RedirectedVirus-SpecificCytotoxicTLymphocytesInVivo"
Caruanaetal.,ClinCancerRes.(2015) -PMID:25691731
"Self-adjuvantedmRNAVaccinationinAdvancedProstateCancerPatients:aFirst-in-ManPhaseI/IIaStudy"
Kübleretal.,JImmunotherCancer.(2015)-PMID:26082837
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"ProfilingTCellActivationUsingSingle-MoleculeFluorescenceInSituHybridizationandFlowCytometry"
Bushkinetal.,J.Immunol.(2015)-PMID:25505292
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK
PepMix™Human(Actin)
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Actin,alphaskeletalmuscle |
| Organism | Homosapiens(Human) |
| Indication/Topic | Control |
| Numberofpeptides | 120 |
FurtherInformation:
| Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | ProtocolandDatasheet |
| Swiss-ProtID | P68133 |
| NCBI | NP_001091 |
| Sequence | MCDEDETTALVCDNGSGLVKAGFAGDDA PRAVFPSIVGRPRHQGVMVGMGQKDSYV GDEAQSKRGILTLKYPIEHGIITNWDDM EKIWHHTFYNELRVAPEEHPTLLTEAPL NPKANREKMTQIMFETFNVPAMYVAIQA VLSLYASGRTTGIVLDSGDGVTHNVPIY EGYALPHAIMRLDLAGRDLTDYLMKILT ERGYSFVTTAEREIVRDIKEKLCYVALD FENEMATAASSSSLEKSYELPDGQVITI GNERFRCPETLFQPSFIGMESAGIHETT YNSIMKCDIDIRKDLYANNVMSGGTTMY PGIADRMQKEITALAPSTMKIKIIAPPE RKYSVWIGGSILASLSTFQQMWITKQEY DEAGPSIVHRKCF |
| ProteinLength(aa) | 377 |
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
