PepMix™Human(Brachyury)
ProductCode:PM-BRAC
Poolof106peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughBrachyuryprotein(Swiss-ProtID:O15178)ofHomosapiensforTcellassays(e.g.ELISPOT).
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):BrachyuryMutation,Cancer,Liver
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
PepMix™Human(Brachyury)
SelectedReferences
"DysfunctionofPSA-specificCD8+TCellsinProstateCancerPatientsCorrelateswithCD38andTim-3Expression"
Jappetal.,CancerImmunolImmunother.(2015)-PMID:26289091
"AnHPV-E6/E7ImmunotherapyPlusPD-1CheckpointInhibitionResultsinTumorRegressionandReductioninPD-L1Expression"
Riceetal.,CancerGeneTherapy(2015)-PMID:26337747
"Survivin-specificCD4+TCellsareDecreasedinPatientswithSurvivin-positiveMyeloma"
Lockeetal.,JImmunotherCancer(2015)-PMID:25992291
"APhaseITrialCombiningDecitabine/dendriticCellVaccineTargetingMAGE-A1,MAGE-A3andNY-ESO-1forChildrenwithRelapsedorTherapy-RefractoryNeuroblastomaandSarcoma"
Krishnadasetal.,CancerImmunolImmunother.(2015)-PMID:26105625
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"PresenceofCirculatingHer-2-reactiveCD8+T-CellsisAssociatedwithLowerFrequenciesofMyeloid-derivedSuppressorCellsandRegulatoryTCells,andBetterSurvivalinElderlyBreastCancerPatients"
KiniBailuretal.,BreastCancerResearch(2015)-PMID:n.a.
"STAT3SignalingContributestotheHighEffectorActivitiesofInterleukin-15-derivedDendriticCells"
Okadaetal.,ImmunolCellBiol.(2015)-PMID:25582338
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
PepMix™Human(Brachyury)
| Application | AntigenspecificT-cellstimulation CancerbioMarkerresearch Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Transcriptionfactors/activators |
| Organism | Homosapiens(Human) |
| Indication/Topic | BrachyuryMutation Cancer |
| Numberofpeptides | 106 |
FurtherInformation:
| Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | ProtocolandDatasheet |
| Swiss-ProtID | O15178 |
| Sequence | MSSPGTESAGKSLQYRVDHLLSAVENEL QAGSEKGDPTERELRVGLEESELWLRFK ELTNEMIVTKNGRRMFPVLKVNVSGLDP NAMYSFLLDFVAADNHRWKYVNGEWVPG GKPEPQAPSCVYIHPDSPNFGAHWMKAP VSFSKVKLTNKLNGGGQIMLNSLHKYEP RIHIVRVGGPQRMITSHCFPETQFIAVT AYQNEEITALKIKYNPFAKAFLDAKERS DHKEMMEEPGDSQQPGYSQWGWLLPGTS TLCPPANPHPQFGGALSLPSTHSCDRYP TLRSHRSSPYPSPYAHRNNSPTYSDNSP ACLSMLQSHDNWSSLGMPAHPSMLPVSH NASPPTSSSQYPSLWSVSNGAVTPGSQA AAVSNGLGAQFFRGSPAHYTPLTHPVSA PSSSGSPLYEGAAAATDIVDSQYDAAAQ GRLIASWTPVSPPSM |
| ProteinLength(aa) | 435 |
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
刚入这行，谢谢大家
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
