请使用支持JavaScript的浏览器! Medchemexpress/Daidzein(Synonyms: Isoflavone)/HY-N0019/10mM*1mL in DMSO_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城
欢迎来到蚂蚁淘！（生命科学科研用品平行进口B2B商城） 请登录 免费注册 询价篮
我的蚂蚁淘
产品分类
网站导航
微信关注
联系QQ
0
热门关键词 jackson二抗 Polyscience 23966 Liposomes脂质体 Biotium溴化乙锭 goldbio荧光素
我的购物车0
全部分类
酶
首页 品牌展示 产品查询 新闻资讯 帮助中心 联系我们 现货平台
商品信息
联系客服
Medchemexpress/Daidzein(Synonyms: Isoflavone)/HY-N0019/10mM*1mL in DMSO
郑重提醒：
无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
Medchemexpress/Daidzein(Synonyms: Isoflavone)/HY-N0019/10mM*1mL in DMSO
品牌 / 
MedChemExpress
货号 / 
HY-N0019-10mM*1mLinDMSO
官网地址
美元价：
(友情提示：该价格仅为参考，欢迎联系客服询价！)
数    量：
免费咨询热线
4000-520-616
商品介绍 售后保障 相关文章 商品咨询
联系客服
Daidzeinisasoyisoflavone,whichactsasaPPARactivator.
Description

Daidzeinisasoyisoflavone,whichactsasaPPARactivator.

IC50&Target

PPAR[1]

InVitro

In3T3-L1ADIpocytes,Daidzeininversestheattenuationofadiponectingeneexpressionbyco-culture,andtheseeffectsareinhibitedbythePPAR-γspecificinhibitor.Daidzeinattenuatesthereductionofadiponectinexpressioninadipocytes,andaPPAR-γspecificinhibitorabrogatedthiseffect.DirectactivationofPPAR-αand-γbyDaidzeinisconfirmedbyaluciferasereporterassay.InHEK293Tcells,DaidzeinsignificantlyincreasesPPAR-αtranscriptionalactivityinaconcentration-dependentmanner.Althoughanobviousdose-dependencyisnotobservedinPPAR-γtranscriptionalactivity,DaidzeinalsosignificantlyincreasesPPAR-γtranscriptionalactivityoverasimilarrangeofconcentrationsatwhichDaidzeinenhancedPPAR-αtranscriptionalactivity,withamaximumincreaseat25μM[1].Daidzeinisasoyisoflavone,whichupregulatestheexpressionofAbcg1,anditpromotesaxonaloutgrowthinculturedhippocampalneuronsviaestrogenreceptorsignaling.Daidzeinisamajorcomponentofsoywithstructuralsimilaritytoestrogen.Itexertsananti-inflammatoryeffect,lowerslipidlevels,andincreasesmitochondrialbiogenesis.Asanactivatorofnuclearreceptorperoxisomeproliferator-activatedreceptors(PPARs),DaidzeinenhancestranscriptionofPPARs-dependentgenes,includingliverXreceptors(LXRs,Nr1hgenefamilyinmice).IncubationwithdifferentconcentrationsofDaidzein,from5to100μM,increasesAPOEtranscriptionalactivity[2].

InVivo

TreatingApoeKOmicewithDaidzeinincreasesLxrandAbca1geneexpressionat1monthafterstroke,showingthattheabsenceofApoEdoesnotinterferewithothercholesterolhomeostasisgeneticprograms.Therefore,thefindingssuggestthatDaidzein-inducedApoEupregulationisacriticalcomponentinfosteringfunctionalrecoveryinchronicstroke[2].

ClinicalTrial
ViewMoreCollapse
References

  • [1].SakamotoY1,etal.TheDietaryIsoflavoneDaidzeinReducesExpressionofPro-InflammatoryGenesthroughPPARα/γandJNKPathwaysinAdipocyteandMacrophageCo-Cultures.PLoSOne.2016Feb22;11(2):e0149676.

    [2].KimE,etal.DaidzeinAugmentsCholesterolHomeostasisviaApoEtoPromoteFunctionalRecoveryinChronicStroke.JNeurosci.2015Nov11;35(45):15113-26.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM3.9333mL19.6665mL39.3329mL
5mM0.7867mL3.9333mL7.8666mL
10mM0.3933mL1.9666mL3.9333mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
CellAssay
[1]

DaidzeinisdissolvedinDMSOandthendilutedwithappropriatemedia[1].

HEK293Tcellsareplatedon24-wellplatesatacelldensityofapproximately2.5×104cells/wellandaregrownto70-80%confluence.CellsarethentransientlytransfectedwithaPPAR-αorPPAR-γexpressionplasmid,andaplasmidcontainingtheluciferasegeneunderthecontrolofthreetandemPPARresponseelements(PPRE×3TK-luciferase)usinganX-tremeGENEHPDNATransfectionReagent.Renillaluciferasecontrolvectorsareco-transfectedtocontrolfortransfectionefficiency.Aftertransfection,cellsareculturedforanother24hinmediumcontainingDMSOorvariousconcentrations(6.25,12.5,25μM)ofDaidzein.Cellsarelysed,andluciferaseactivityismeasuredandexpressedasfoldinduction,thatisnormalizedtotheactivityoftherenillaluciferasecontrolplasmid[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

AnimalAdmiNISTration
[2]

Mice[2]
Experimentsareperformedin10-to11-week-oldmaleC57(C57bl/6)andApoeKO(C57background)mice.Forlong-termstrokerecovery,micereceiveMoxifloxacin(100mg/kg)for3d.Theprophylacticantibiotictreatmentisshowntoeffectivelyreducemortalityinananimalmodelofstrokebyattenuatingperipheralinfection.Inaddition,salineissubcutaneouslyadministereddaily,andhydrogel(ClearH2O)isgiventopreventdehydration.Withtheimplementationofpoststrokecare(antibioticregimen,rehydration,andfeedinghydrogelswithsoftdiet)duringtheacuteperiod(<1 week),="" mice="" start="" to="" regain="" their="" body="" weight="" by="" day="" 5="" and="" continue="" to="" recover="" from="" stroke.="" animals="" are="" randomly="" selected="" for="" vehicle="" or="" daidzein="" treatment.="" vehicle="" or="" daidzein="" (10="" mg/kg)="" is="" administered="" subcutaneously="" within="" 30="" min="" of="" reperfusion="" after="" confirming="" the="" reperfusion="" of="" blood="" flow,="" daily="" for="" 7="" d="" and="" then="" every="" other="" day="" up="" to="" 1="" month.="" mce="" has="" not="" independently="" confirmed="" the="" accuracy="" of="" these="" methods.="" they="" are="" for="" reference="">

References

  • [1].SakamotoY1,etal.TheDietaryIsoflavoneDaidzeinReducesExpressionofPro-InflammatoryGenesthroughPPARα/γandJNKPathwaysinAdipocyteandMacrophageCo-Cultures.PLoSOne.2016Feb22;11(2):e0149676.

    [2].KimE,etal.DaidzeinAugmentsCholesterolHomeostasisviaApoEtoPromoteFunctionalRecoveryinChronicStroke.JNeurosci.2015Nov11;35(45):15113-26.

MolecularWeight

254.24

Formula

C₁₅H₁₀O₄

CASNo.

486-66-8

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

10mMinDMSO

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:99.69%

匿名提交