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Medchemexpress/Hygromycin B(Synonyms: Hygrovetine)/HY-B0490/10mM*1mL in Water
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Medchemexpress/Hygromycin B(Synonyms: Hygrovetine)/HY-B0490/10mM*1mL in Water
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HygromycinBisanaminoglycosideantibioticactiveagainstprokaryoticandeukaryoticcells.
Description

HygromycinBisanaminoglycosideantibioticactiveagainstprokaryoticandeukaryoticcells.

IC50&Target

Target:Antibacterial;Antifungal[1]

InVitro

HygromycinB,anaminocyclitolantibioticthatstronglyinhibitsboth70Sand80Sribosomes,issynthesizedbyStreptomyceshygroscopicus[1].HygromycinBat0.38mMconcentrationcompletelyhaltsyeastcellgrowthinrichmedia,presumablybypreventingproteinsynthesisbycytoplasmicribosomes.Polypeptidesynthesisincell-freeextractsfromrabbitreticulocytes,wheatgermandyeastisstronglyblockedbylowconcentrationsofhygromycinB.TheantibioticinhibitspeptidechainelongationbyyeastpolysomesbypreventingelongationfactorEF-2-dependenttranslocation.TheinhibitionoftranslocationbyhygromycinBmightresultfromthestABIlizationofpeptidyl-tRNAboundtotheribosomalacceptorsite[2].

InVivo

HygromycinBinhibitsproteinsynthesisbyblockingribosomaltranslocationwithoutcausingsignificantmisreADInginvivo[3].ConstitutiveexpressionofthebacterialhygRgeneintransgenicmiceinvivoconfersresistancetohygromycinB[4].

References
  • [1].PardoJM,etal.Biochemicalbasisof resistance to hygromycin B inStreptomyceshygroscopicus--theproducingorganism.JGenMicrobiol. 1985Jun;131(6):1289-98.

    [2].GonzálezA,etal.StudiesonthemodeofactionofhygromycinB,aninhibitoroftranslocationineukaryotes.BiochimBiophysActa.1978Dec21;521(2):459-69.

    [3].PfisterP,etal.Roleof16SrRNAHelix44inRibosomalResistancetoHygromycinB.AntimicrobAgentsChemother.2003May;47(5):1496-502.

AnimalAdmiNISTration
[4]

HygromycinBisdissolvedinsterilewater.ThemiceC57BL/6J-TgN(pPWL512hyg)1EmscarryinghygRaretreatedwithasingledoseofhygromycinBi.p.atdosesthatstartsat2.7mg/kgandincreasesby50%foreachconsecutivedose.Controlwild-typeC57BL/6Jmicearetreatedwiththesamevolumeofsterilesaline.Totalvolumeinjectedis0.5mL.Thehealthstatusandbodyweightsofanimalsaremonitoreddailyfor10consecutivedays[4].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References
  • [1].PardoJM,etal.Biochemicalbasisof resistance to hygromycin B inStreptomyceshygroscopicus--theproducingorganism.JGenMicrobiol. 1985Jun;131(6):1289-98.

    [2].GonzálezA,etal.StudiesonthemodeofactionofhygromycinB,aninhibitoroftranslocationineukaryotes.BiochimBiophysActa.1978Dec21;521(2):459-69.

    [3].PfisterP,etal.Roleof16SrRNAHelix44inRibosomalResistancetoHygromycinB.AntimicrobAgentsChemother.2003May;47(5):1496-502.

MolecularWeight

527.52

Formula

C₂₀H₃₇N₃O₁₃

CASNo.

31282-04-9

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

H2O:≥30mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:>98.00%