Pilocarpine HCl is a nonselective muscarinic acetylcholine receptor agonist used to produce an experimental model of epilepsy.
Description
Pilocarpine HCl is a nonselective muscarinic acetylcholine receptor agonist used to produce an experimental model of epilepsy.
Target: mAChR
Pilocarpine is a parasympathomimetic alkaloid obtained from the leaves of tropical American shrubs from the genus Pilocarpus. It is a non-selective muscarinic receptor agonist in the parasympathetic nervous system, which acts therapeutically at themuscarinic acetylcholine receptor M3 due to its topical application, e.g., in glaucoma and xerostomia. Pilocarpine acts on a subtype of muscarinic receptor (M3) found on the iris sphincter muscle, causing the muscle to contract and engage in miosis [1]. Pilocarpine also acts on the ciliary muscle and causes it to contract. When the ciliary muscle contracts, it opens the trabecular meshwork through increased tension on the scleral spur. This action facilitates the rate that aqueous humorleaves the eye to decrease intraocular pressure. In ophthalmology, pilocarpine is also used to reduce the possibility of glare at night from lights when the patient has undergone implantation of phakic intraocular lenses; the use of pilocarpine would reduce the size of the pupils, relieving these symptoms. The most common concentration for this use is pilocarpine 1%, the weakest concentration. Pilocarpine is also used to treat dry mouth (xerostomia) which can occur, for example, as a side effect of radiation therapy for head and neck cancers. Pilocarpine stimulates the secretion of large amounts of saliva and sweat [2].
Clinical Trial
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References
[1]. Maslanski, J.A., et al., Assessment of the muscarinic receptor subtypes involved in pilocarpine-induced seizures in mice. Neurosci Lett, 1994. 168(1-2): p. 225-8.
[2]. Mayorga, A.J., et al., Characterization of the muscarinic receptor subtype mediating pilocarpine-induced tremulous jaw movements in rats. Eur J Pharmacol, 1999. 364(1): p. 7-11.
Preparing Stock Solutions
ConcentrationVolumeMass
1 mg
5 mg
10 mg
1 mM
4.0863 mL
20.4315 mL
40.8630 mL
5 mM
0.8173 mL
4.0863 mL
8.1726 mL
10 mM
0.4086 mL
2.0432 mL
4.0863 mL
Please refer to the solubility information to select the appropriate solvent.
References
[1]. Maslanski, J.A., et al., Assessment of the muscarinic receptor subtypes involved in pilocarpine-induced seizures in mice. Neurosci Lett, 1994. 168(1-2): p. 225-8.
[2]. Mayorga, A.J., et al., Characterization of the muscarinic receptor subtype mediating pilocarpine-induced tremulous jaw movements in rats. Eur J Pharmacol, 1999. 364(1): p. 7-11.
Molecular Weight
244.72
Formula
C₁₁H₁₇ClN₂O₂
CAS No.
54-71-7
Storage
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
Shipping
Room temperature in continental US; may vary elsewhere
[1]. Maslanski, J.A., et al., Assessment of the muscarinic receptor subtypes involved in pilocarpine-induced seizures in mice. Neurosci Lett, 1994. 168(1-2): p. 225-8.
[2]. Mayorga, A.J., et al., Characterization of the muscarinic receptor subtype mediating pilocarpine-induced tremulous jaw movements in rats. Eur J Pharmacol, 1999. 364(1): p. 7-11.
Single Primer ("Semi-Random") PCRJuly 26, 2000 ECKDescriptionSingle primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmi 查看更多>
