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Medchemexpress/5-BrdU(Synonyms: BUdR; BRDU)/HY-15910/10mM*1mL in DMSO
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Medchemexpress/5-BrdU(Synonyms: BUdR; BRDU)/HY-15910/10mM*1mL in DMSO
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5-BrdUisanucleosideanalog,andcompeteswiththymidineforincorporationintoDNA,andisusedinthedetectionofproliferatingcells.

CustomerValidation

  • Obesity(SilverSpring).2017Sep;25(9):1532-1539.
  • AmJTranslRes.2016Sep15;8(9):3955-3962.
  • AmJTranslRes.2016Jun15;8(6):2562-74.
Description

5-BrdUisanucleosideanalog,andcompeteswiththymidineforincorporationintoDNA,andisusedinthedetectionofproliferatingcells.

InVitro

Bromodeoxyuridineinducesaprogressive,dose-responsivesuppressionofcancercelllineandcancerstemcellpopulationexpansionRG2ratgliomacells.InH9cellsandBJfibroblasts,bromodeoxyuridinealtersthecellcycleprofile[1]. BrdUisstablyintegratedintotheDNA,andthuscanbeusedinassessmentofcellproliferationandothercellprocession[2]

InVivo

Bromodeoxyuridine(300mg/kg,i.p.or0.8mg/mL,p.o.)significantlyslowstumorprogressioninratgliomaRG2tumormodel[1].

ClinicalTrial
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References
  • [1].LevkoffLH,etal.Bromodeoxyuridineinhibitscancercellproliferationinvitroandinvivo.Neoplasia.2008Aug;10(8):804-16.

    [2].RothaeuslerK,etal.Assessmentofcellproliferationby5-bromodeoxyuridine(BrdU)labelingformulticolorflowcytometry.CurrProtocCytom.2007Apr;Chapter7:Unit7.31.

PreparingStockSolutions
ConcentrationVolumeMass1mg5mg10mg
1mM3.2563mL16.2813mL32.5627mL
5mM0.6513mL3.2563mL6.5125mL
10mM0.3256mL1.6281mL3.2563mL
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
CellAssay
[1]

Culturesareinitiallyplatedat2000cells/cm2 andarequantifiedwithaZ2CoulterCounter.RG2ratgliomacellsaretreatedoncewith0,1,10,or50µMBrdUfor24hours,andcumulativegrowthcurvesareobtainedover18days.Controlandtreatedcellsarequantifiedandreplatedatequaldensitiesondays5,12,and18aftertreatment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References
  • [1].LevkoffLH,etal.Bromodeoxyuridineinhibitscancercellproliferationinvitroandinvivo.Neoplasia.2008Aug;10(8):804-16.

    [2].RothaeuslerK,etal.Assessmentofcellproliferationby5-bromodeoxyuridine(BrdU)labelingformulticolorflowcytometry.CurrProtocCytom.2007Apr;Chapter7:Unit7.31.

MolecularWeight

307.1

Formula

C₉H₁₁BrN₂O₅

CASNo.

59-14-3

Storage
Powder-20°C3years
 4°C2years
Insolvent-80°C6months
 -20°C1month
Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:≥41mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:98.93%