Medchemexpress/Metformin hydrochloride(Synonyms: 1,1-Dimethylbiguanide hydrochloride)/HY-17471A/10mM*1mL in Water_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall 蚂蚁淘商城

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Medchemexpress/Metformin hydrochloride(Synonyms: 1,1-Dimethylbiguanide hydrochloride)/HY-17471A/10mM*1mL in Water

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Medchemexpress/Metformin hydrochloride(Synonyms: 1,1-Dimethylbiguanide hydrochloride)/HY-17471A/10mM*1mL in Water

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MedChemExpress

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HY-17471A-10mM*1mLinWater

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4000-520-616

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Metformin(hydrochloride)isafirst-linedrugforthetreatmentoftype2diabetesandthereisincreasingevidenceofapotentialefficacyofthisagentasananti-cancerdrug.

CustomerValidation

•MolCellProteomics.2017Jul;16(7):1324-1334.
•MolNeurobiol.2016May11.
•MolOncol.2017Aug;11(8):1035-1049.
•CellPhysiolBiochem.2017Nov30;44(4):1381-1395.
•BiochemPharmacol.2017Aug15;138:49-60.
•JCellMolMed.2017Jul12.

•FrontPharmacol.2016Oct20;7:390.

Description

Metformin(hydrochloride)isafirst-linedrugforthetreatmentoftype2diabetesandthereisincreasingevidenceofapotentialefficacyofthisagentasananti-cancerdrug.

InVitro

MetformininhibitsproliferationofESCsinaconcentration-dependentmanner.TheIC₅₀is2.45 mMforA-ESCsand7.87 mMforN-ESCs.MetforminshowspronouncedeffectsonactivationofAMPKsignalinginA-ESCsfromsecretoryphasethanincellsfromproliferativephase^[3].Metformin(0-500μM)decreasesglycogensynthesisinadose-dependentmannerwithanIC₅₀valueof196.5μMinculturedrathepatocytes^[4].MetforminshowscellviABIlityandcytotoxiceffectsonPC-3cellswithIC₅₀of5mM^[5].

InVivo

Metformin(100mg/kg,p.o.)alone,andmetformin(25,50,100mg/kg)withisoproterenolgroupsattenuatesmyocytenecrosisthroughhistopathologicalanalysis^[1].Metformin(>900mg/kg/day,p.o.)resultsinmoribundity/mortalityandclinicalsignsoftoxicityinCrl:CD(SD)rats^[2].

ClinicalTrial

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References

[1].SorayaH,etal.Acutetreatmentwithmetforminimprovescardiacfunctionfollowingisoproterenolinducedmyocardialinfarctioninrats.PharmacolRep.2012;64(6):1476-84.
[2].QuaileMP,etal.Toxicityandtoxicokineticsofmetformininrats.ToxicolApplPharmacol.2010Mar15;243(3):340-7.
[3].XueJ,etal.MetformininhibitsgrowthofeutopicstromalcellsfromadenomyoticendometriumviaAMPKactivationandsubsequentinhibitionofAKTphosphorylation:apossIBLeroleinthetreatmentofadenomyosis.Reproduction.2013Aug21;146(4):397-406.
[4].OttoM,etal.Metformininhibitsglycogensynthesisandgluconeogenesisinculturedrathepatocytes.DiabetesObesMetab.2003May;5(3):189-94.
[5].AvciCB,etal.Therapeuticpotentialofananti-diabeticdrug,metformin:alterationofmiRNAexpressioninprostatecancercells.AsianPacJCancerPrev.2013;14(2):765-8.
[6].NieL,etal.TheLandscapeofHistoneModificationsinaHigh-FatDiet-InducedObese(DIO)MouseModel.MolCellProteomics.2017Jul;16(7):1324-1334.
[7].ZhangD,etal.MetforminamelioratesBSCBdisruptionbyinhibitingneutrophilinfiltrationandMMP-9expressionbutnotdirectTJproteinsexpressionregulation.JCellMolMed.2017Jul12.

PreparingStockSolutions

ConcentrationVolumeMass	1mg	5mg	10mg

1mM	6.0379mL	30.1896mL	60.3792mL
5mM	1.2076mL	6.0379mL	12.0758mL
10mM	0.6038mL	3.0190mL	6.0379mL

Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.

CellAssay
^[3]

Metforminisdissolvedindouble-distilledwateratastockconcentrationof10mMandstoredat4°C.

ESCsareplatedin96-wellplatesataconcentrationof1×10³cells/well.Afterattachment,cellsaretreatedwithdifferentdosesofmetformin/compoundCfor0min,15min,1h,and24h.MTTassaysareperformedasdescribedpreviously.Inbrief,MTT(5mg/mL)isaddedtothe96-wellplatesatavolumeof10μL/well,andtheplatesareincubatedfor4h.TheMTTreactionisterminatedbyremovaloftheculturemediumcontainingMTT,and100μLDMSOperwellareaddedandincubatedatRTonashakerfor10mintoensurethatthecrystalshaddissolvedsufficiently.Absorbancevaluesaremeasuredat595nm.Cellproliferation(percentageofcontrol)iscalculatedasfollows:absorbance(experimentalgroup)/absorbance(controlgroup).Cellproliferationinhibition(percentageofcontrol)iscalculatedasfollows:100%−cellproliferation(percentageofcontrol).Eachexperimentisperformedinduplicateandrepeatedsixtimestoassessresultconsistency.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

AnimalAdmiNISTration
^[1]

Metformin(hydrochloride)isdissolvedinphysiologicalsaline.

Theanimalsarerandomizedintosixgroupsconsistingofsixratseach.Ratsingroup1(control)receivesasubcutaneousinjectionofphysiologicalsaline(0.5mL)andareleftuntreatedfortheentireexperimentalperiod.Ratsingroup2receivesanoraladministrationofmetformin(100mg/kg;twicedaily)for2daysandaresubcutaneouslyinjectedwithsalineatanintervalof24hfor2consecutivedays.Ratsingroup3(MIcontrol)receivesanoraladministrationofsaline(twicedaily)for2daysandarescinjectedwithisoproterenol(100mg/kg)dailyfor2consecutivedaysatanintervalof24h.Ratsingroups4to6aretreatedwithmetforminat25,50,and100mg/kg.Metforminisdissolvedinsalineandisgavagedatavolumeof0.25-0.5mLtwiceadayatanintervalof12h,startedimmediatelybeforeisoproterenolinjection.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

[1].SorayaH,etal.Acutetreatmentwithmetforminimprovescardiacfunctionfollowingisoproterenolinducedmyocardialinfarctioninrats.PharmacolRep.2012;64(6):1476-84.
[2].QuaileMP,etal.Toxicityandtoxicokineticsofmetformininrats.ToxicolApplPharmacol.2010Mar15;243(3):340-7.
[3].XueJ,etal.MetformininhibitsgrowthofeutopicstromalcellsfromadenomyoticendometriumviaAMPKactivationandsubsequentinhibitionofAKTphosphorylation:apossibleroleinthetreatmentofadenomyosis.Reproduction.2013Aug21;146(4):397-406.
[4].OttoM,etal.Metformininhibitsglycogensynthesisandgluconeogenesisinculturedrathepatocytes.DiabetesObesMetab.2003May;5(3):189-94.
[5].AvciCB,etal.Therapeuticpotentialofananti-diabeticdrug,metformin:alterationofmiRNAexpressioninprostatecancercells.AsianPacJCancerPrev.2013;14(2):765-8.
[6].NieL,etal.TheLandscapeofHistoneModificationsinaHigh-FatDiet-InducedObese(DIO)MouseModel.MolCellProteomics.2017Jul;16(7):1324-1334.
[7].ZhangD,etal.MetforminamelioratesBSCBdisruptionbyinhibitingneutrophilinfiltrationandMMP-9expressionbutnotdirectTJproteinsexpressionregulation.JCellMolMed.2017Jul12.

MolecularWeight

165.62

Formula

C₄H₁₂ClN₅

CASNo.

1115-70-4

Storage

Powder	-20°C	3years
	4°C	2years
Insolvent	-80°C	6months
	-20°C	1month

Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

H₂O:≥32mg/mL;DMSO:≥1.7mg/mL

Metforminhydrochlorideispreparedin0.5%methylcellulose^[6].
Metforminhydrochlorideisdilutedwithnormalsaline,toachieveafinalmetforminconcentrationof20mg/mL^[7].

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

References

[1].SorayaH,etal.Acutetreatmentwithmetforminimprovescardiacfunctionfollowingisoproterenolinducedmyocardialinfarctioninrats.PharmacolRep.2012;64(6):1476-84.
[2].QuaileMP,etal.Toxicityandtoxicokineticsofmetformininrats.ToxicolApplPharmacol.2010Mar15;243(3):340-7.
[3].XueJ,etal.MetformininhibitsgrowthofeutopicstromalcellsfromadenomyoticendometriumviaAMPKactivationandsubsequentinhibitionofAKTphosphorylation:apossibleroleinthetreatmentofadenomyosis.Reproduction.2013Aug21;146(4):397-406.
[4].OttoM,etal.Metformininhibitsglycogensynthesisandgluconeogenesisinculturedrathepatocytes.DiabetesObesMetab.2003May;5(3):189-94.
[5].AvciCB,etal.Therapeuticpotentialofananti-diabeticdrug,metformin:alterationofmiRNAexpressioninprostatecancercells.AsianPacJCancerPrev.2013;14(2):765-8.
[6].NieL,etal.TheLandscapeofHistoneModificationsinaHigh-FatDiet-InducedObese(DIO)MouseModel.MolCellProteomics.2017Jul;16(7):1324-1334.
[7].ZhangD,etal.MetforminamelioratesBSCBdisruptionbyinhibitingneutrophilinfiltrationandMMP-9expressionbutnotdirectTJproteinsexpressionregulation.JCellMolMed.2017Jul12.

Purity:99.08%

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