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GenWay/Dehydroepiandrosterone-Sulfate ELISA Kit/GWB-46B3CF/96 TESTS
免费咨询热线
4000-520-616
Description:
| ProductName | Dehydroepiandrosterone-Sulfate |
|---|---|
| Category | Fertility. |
| SampleType | Serumandplasma. |
| Sensitivity | 0.044ug/mL. |
| Description | DHEA-SEnzymeImmunoassayKitprovidesmaterialsfortheQuantitativeDeterminationofDHEA-Sinserumandplasma. |
| Introduction | TheGenWayDHEA-SEnzymeImmunoassayKitprovidesmaterialsforthequantitativedeterminationofDHEA-Sinserumandplasma. Thisassayisintendedforresearchuseonly. Dehydroepiandrosterone(5-Androstene-3β-0L-17-one,Androstenolone,Dehydroisoandrosterone,Transdehydroandrosterone,DHEA)isasteroidhormonepresentinbloodmostlyinitssulfateform(DHEA-S). DHEA-Sisamorespecificproductoftheadrenalsandmeasurementsofthissteroidarewidelyusedinclinicalpractice.TheclinicalimportanceofplasmaassaysofDHEA-Sisassociatedwiththediagnosisofadrenalhyperplasiaanddifferentialdiagnosisofhirsutism(1). |
| PrincipleoftheTest | TheGenWayDHEA-SELISAKitisasolidphaseenzyme-linkedimmunosorbentassay(ELISA),basedontheprincipleofcompetitivebinding.ThemicrotiterwellsarecoatedwithapolyclonalantibodydirectedtowardsauniqueantigenicsiteoftheDHEA-Smolecule. EndogenousDHEA-SofapatientsamplecompeteswithaDHEA-ShorserADIshperoxidaseconjugateforbindingtothecoatedantibody.Afterincubationtheunboundconjugateiswashedoff. TheamountofboundperoxidaseconjugateisreverseproportionaltotheconcentrationofDHEA-Sinthesample.Afteradditionofthesubstratesolution,theintensityofcolourdevelopedisreverseproportionaltotheconcentrationofDHEA-Sinthepatientsample. |
| StorageandStABIlity | Whenstoredat2°C-8°Cunopenedreagentswillretainreactivityuntilexpirationdate.Donotusereagentsbeyondthisdate. Allopenedreagentsmustbestoredat2°C-8°C.Microtiterwellsmustbestoredat2°C-8°C.Oncethefoilbaghasbeenopened,careshouldbetakentocloseittightlyagain. |
| Function | Catalyzesthesulfationofsteroidsandbileacidsintheliverandadrenalglands. |
| CatalyticActivity | 3"-phosphoadenylylsulfate+glycolithocholate=adenosine3",5"-bisphosphate+glycolithocholate3-sulfate 3"-phosphoadenylylsulfate+taurolithocholate=adenosine3",5"-bisphosphate+taurolithocholatesulfate. |
| Subunit | Homodimer. |
| SubcellularLocation | Cytoplasm. |
| TissueSpecificity | Liver,adrenalandatlowerlevelinthekidney.Ispresentinhumanfetusinhigherlevelintheadrenalthantheliverandthekidney. |
| Ptm | TheN-terminusisblocked. |
| Miscellaneous | Estrogenspresentinmaternalcirculationispredominantlyderivedfromfetaldehydroepiandosteronesulfatewhichishydrolyzedandmetabolizedtoestrogensinplacenta. |
| Similarity | Belongstothesulfotransferase1family. |
AdditionalInformation:
| Name | Dehydroepiandrosterone-SulfateELISAKit |
|---|---|
| RelatedProductNames | Dehydroepiandrosterone-SulfateELISAKit;EC2.8.2.14;HydroxysteroidSulfotransferase;HST;Dehydroepiandrosteronesulfotransferase;DHEA-ST;ST2;ST2A3HST;STDDehydroepiandrosterone-SulfateDHEA-S |
| NCBIAccNumber | NP_003158.2 |
| MolecularWeight | 33780 |
| SwissProtNumber | Q06520 |
| IntendedUse | ResearchUseOnly |

