- SynonymIL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
- SourceBiotinylated Human IL-17A&IL-17F, Avitag,His Tag (ILF-H82W1) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)).Predicted N-terminus: Gly 24Request for sequence
- Molecular Characterization
Biotinylated Human IL-17A&IL-17F, Avitag,His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 34.1 kDa. Subunit IL-17A&IL-17F is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The reducing (R) protein migrates as 40-43 kDa due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-17A&IL-17F, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-Human IL17A MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17A&IL-17F, Avitag,His Tag (Cat. No. ILF-H82W1) with a linear range of 0.2-6 ng/mL (QC tested).
- BackgroundInterleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
- References
- (1)Yao Z., et al.,1995, J. Immunol. 155:5483-5486.
- (2)Gerhardt S., et al., 2009, J. Mol. Biol. 394:905-921.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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近期根据经验总结了原代肿瘤细胞分离培养的2个关键因素。
1、组织的活性：如果我们取的都是坏死的组织或者结缔组织，我相信任何方法都不可能分离出来活的细胞的。另外，取完的组织要冷藏存放，并且越早分离越好，最好在24h内，最久不要超过48h。
2、组织的消化：在组织消化前，组织剪的越碎越好（1mm3左右为宜），组织的大小决定了组织的消化时间，不同组织消化时间差别较大：例如肺癌在2h-2.5h；食管癌在1个多小时。
希望可以帮到各位研友，另外如果大家有更好的经验可以一起来讨论！
请大神前辈指点指点，已经研三了，实验很不顺利，再这样都要延期的节奏了。请问最近急性分离出来的大鼠心肌细胞很不耐钙，做膜片钳封接不上可能是什么原因造成的？谢谢了！
我做的临床试验，就想简单检测PBMC细胞的凋亡，上流式检测，有啥检测效果好的步骤推荐一下，谢谢
各位师兄师姐：
我近期负责的课题，需要进行人类血液淋巴细胞和DC细胞的分离和培养，但我在方面懂的不多，想求助这方面的大神们。后续我需要做RT-qPCR，western-blot、免疫荧光等实验，我应该去多少ml全血比较好？全血怎么保存？怎么进行分离？后续怎么培养和保存？
谢谢！
我的步骤：
1、16－22天小鼠颈椎脱臼法处死后，75%酒精浸泡30秒消毒，睾丸取出后置预冷PBS，40分钟（路上所需时间）后开始处理。
2、剔除白膜，剪碎睾丸。0.25%胰酶（含edta）1.5ml/个睾丸37°C消化30-50分钟，等体积DMEM/F12含10%FBS终止消化，1000r/MIN离心5分钟，弃上液。
3、加0.1%胶原酶消化30分钟，过200目网筛，1000r/MIN离心5分钟，弃上液。
4、DMEM/F12含10%FBS5ml重悬后转如培养瓶，入孵育箱37°C5%CO2培养。
请教各位，我到底是哪里有问题，是胰酶消化的时间长了吗？
想用二步酶消法来分离毛乳头细胞，一个毛囊大概有多少个毛乳头细胞？处于生长期的毛囊在显微镜下有什么特点？先谢谢大神们指点
大概步骤如下：c57小鼠大概六七只，酒精浸泡后不处死取出，迅速打开胸腔取出心脏放入冰PBS冲洗。待心脏取出完毕，冲洗移入有PBS青霉素小瓶中用眼科剪剪成较小组织块。
之后加入0.1％胰酶消化（之前浓度为0.25％，活细胞数量更少。）吹打1min.放入孵箱中3min.弃去上清，加入∥型胶原酶（0.1％）吹打10Min，放入孵箱15Min。取出后加入1ml胰酶。吹打1Min后加入含血清F12培养液终止消化，800转离心5Min。之后取沉淀重悬种板。90Min差速贴壁法纯化心肌细胞。
48h后只有少量（个位数）细胞贴壁，呈三角形。
第一次用0.25％胰酶时离心后有大量鼻涕样物质。
第二次胰酶浓度减小后没有，但细胞沉淀少，且为半透明微白。是不是离心时间不够。
感觉细胞整体状态都不好，几次都没有大量心肌细胞，更不用说观察到心肌细胞搏动。
老师们能不能帮忙分析一下是哪里的原因呢。
有没有比较稳定的小鼠乳鼠心肌细胞分离步骤呢。
希望大神快点出现。
经过无数次的大鼠肝脏插针灌注，忍受过实验结果不理想的打击和折磨，今天终于成功了，特分享些相关图片以鼓励还在奋战中的网友们。
