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TCEP(tris(2-carboxyethyl)phosphine)isastrongreducingagent.Comparedtodithiothreitol(DTT)andβ-mercaptoethanol,TCEPhastheadvantagesofbeingodorless,amorepowerfulreducingagent,anirreversIBLereducingagent,morehydrophilic,andmoreresistanttooxidationinair.ItissolubleinwaterandstableatneutralpH.ItdoesnotreducemetalsusedinimmobilizedmetalaffinitychromatographysuchasNi-NTAresin.TCEPisparticularlyusefulwhenlabelingcysteineresidueswithmaleimides.
Note:Make40mL1MTCEPstocksolution
1.Weigh11.47gTCEPpowder.Dissolvein25mlmolecularBIOLOGygradewater(stirringorshakingmaybenecessary).Theresultingsolutionisat~pH2.5.
2.BringthesolutiontopH7.0with10NNaOH(preparedinmolecularbiologygradewater).
3.Bringtoafinalvolumeof40mlwithmolecularbiologygradewater.
4.Aliquotandstoreat-20°C.
Note:Make40mL1MTCEPstocksolution
1.Weigh11.47gTCEPpowder.Dissolvein25mlmolecularBIOLOGygradewater(stirringorshakingmaybenecessary).Theresultingsolutionisat~pH2.5.
2.BringthesolutiontopH7.0with10NNaOH(preparedinmolecularbiologygradewater).
3.Bringtoafinalvolumeof40mlwithmolecularbiologygradewater.
4.Aliquotandstoreat-20°C.
AdditionalInformation
ProductName: | TCEP |
---|---|
AlsoKnownAs: | Tris(2-Carboxyethyl)phosphineHydrochloride |
CatalogNo.: | P1020-25 |
Size: | 25g |
CAS: | 51805-45-9 |
Formula: | C9H15O6P∙HCl |
MolecularWeight: | 286.65Da |
Form: | Lyophilizedpowder |
QualityAssurance: | >95%byHPLC |
Source: | Synthetic |
Storage: | Eligibleforroomtemperatureshipping.Storeat2-8°Cuponreceiving |
PDFDataSheet: | DownloadPDFdatasheet |
Image(s): | N/A |
ShippingMethod: | Roomtemperatureshipping |
References: | N/A |