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ubpbioHighCapacityStreptavidinAgaroseResinisanaffinitychromatographymediumdesignedforeasy,one-steppurificationofbiotinylatedpeptides,antibodies,lectins,etcfromsamples.Thepurifiedrecombinantstreptavidiniscovalentlycouplingto4%highlycross-linkedagarose.Thecouplingisoptimizedtogivehighbindingcapacityforbiotinylatedmolecules.ThetotalbindingcapacityofStreptavidinResinismorethan120nmolofD-Biotin/mlsettledresin.
Streptavidinisabiotin-bindingproteinfoundintheculturebrothofthebacteriumStreptomycesavidinii.Streptavidinbinds4molesofbiotinpermoleofproteinwithanextremelyhighaffinity.Streptavidinlackscarbohydratesidechainspresentonavidinandhasanisoelectricpointof6.5(vs10foravidin)closetowheremostusefulBIOLOGicalinteractionsoccur.Asaresult,streptavidinfrequentlyexhibitsmuchlowernon-specificbindingthanavidindoes.
Streptavidinisabiotin-bindingproteinfoundintheculturebrothofthebacteriumStreptomycesavidinii.Streptavidinbinds4molesofbiotinpermoleofproteinwithanextremelyhighaffinity.Streptavidinlackscarbohydratesidechainspresentonavidinandhasanisoelectricpointof6.5(vs10foravidin)closetowheremostusefulBIOLOGicalinteractionsoccur.Asaresult,streptavidinfrequentlyexhibitsmuchlowernon-specificbindingthanavidindoes.
AdditionalInformation
ProductName: | HighCapacityStreptavidinAgaroseResin |
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CatalogNo.: | P3070-5 |
Size: | 5ml |
Bead(Geometry,size): | 90μm(45-165μm) |
Cross-Linked: | Yes |
Ligand: | Streptavidin |
Agarose%: | 4%Agarose |
BindingCapacity: | Morethan120nmolofD-biotinpermlofcolumn-volume |
Volume%: | 50%(v/v)aqueoussUSPendedin1XPBScontaining20%ethanol |
StorageTemperature: | 2-8°C |
Application: | Batch,Gravity |
PDFDataSheet: | No |
User"sManual: | Datasheet,User’sG |
Image(s): | No |
ShippingMethod: | Weticeshipping |