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DextrinXPureAgaroseResinisachromatographymediumfortheisolationofproteinsfusedtomaltosebindingprotein(MBP-taggedprotein).TaggingproteinswithMBPoftengivesincreasedexpressionlevelsandhighersolubilityofthetargetprotein.ProperfoldingoftheattachedproteinhasalsobeenshowntobepromotedbytheMBPtag.SinceMBPincreasesolubility,thetagisparticularlyusefulforrecombinantproteinsaccumulatedinaninsolubleform(inclusionbodies).Seetable1.AffinitypurificationusingDextrinXPureAgaroseResintakeplaceunderphysiologicalconditionsandmildelutionisperformedusingmaltosewhichpreservetargetproteinactivity.
AdditionalInformation
| ProductName: | DextrinXPureAgaroseResin |
|---|---|
| CatalogNo.: | P3080-50 |
| Size: | 50ml |
| Bead(Geometry,size): | 45-165µm |
| Cross-Linked: | Yes |
| Ligand: | Dextrin |
| Agarose%: | Highlycross-linked6%agarose |
| BindingCapacity: | >10mgMBPtaggedprotein(80kDa) |
| Volume%: | 50%(v/v)aqueoussUSPensioncontaining20%Ethanol |
| StorageTemperature: | 2-8°C |
| Application: | Batch,Gravity,MPLCandFPLC |
| PDFDataSheet: | PDFDatasheet |
| User"sManual: | PDFUser’sGuide |
| Image(s): | No |
| ShippingMethod: | Weticeshipping |

