ProductHighlights:
TruSeqSmallRNALibraryPreparationKitsprovidereagentstogeneratesmallRNAlibrariesdirectlyfromtotalRNA.MicroRNAs(miRNAs)generatedbyDicerprocessingareefficientlytargetedbytheincludedmodifiedadapters.
Thesekitsenablemultiplexedsequencingwiththeintroductionof48uniqueindexes,allowingmiRNAandsmallRNAdiscoveryandprofilingthroughputtomatchtheunparalleledoutputofIlluminasequencing.Indexesareaddedinauniversalamplificationreaction,greatlyreducingligationbiasandensuringaccuratemeasurementofmiRNAexpression.
Workflowimprovementsenablestreamlinedsamplepreparation,allowingeconomicalstudiescoveringallsmallRNAtranscriptsinanyspecies.CompatIBLeapplicationsincludefindingnovelmiRNAs,characterizingvariationsuchasisomerswithsingle-baseresolution,andanalyzingdifferentialexpressionwithoutpriorassumptions.
TruSeqSmallRNASampleDatasets
TheHumanBrainReferenceRNA(HBRR)andUniversalHumanReferenceRNA(UHRR)sampleswerepreparedusingtheTruSeqSmallRNALibraryPrepKit.TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x36bpreadlengthconfiguration.Thetotalyieldwas0.91Gbwith97%ofbasesatoraboveQ30.
BrowsetheDataInBaseSpaceSequenceHub:
- ViewRun(primaryanalysisandmetrics)
- ViewProject(secondaryanalysiswithBaseSpaceApps)
Specifications:
| AssayTime | ~10.5hours |
| Hands-OnTime | ~30minutes |
| InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
| ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
| MechanismofAction | Oligo-dTbeadscapturepolyAtails |
| Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
| SampleThroughput | Upto16samplesperNeoPreplibrarycard |
| SpeciesCategory | Drosophila,AnySpecies,Mouse,Zebrafish,Human,Rat,Plant,Nematode |
| SystemCompatibility | MiSeq,NextSeq550,MiniSeq,NextSeq500 |
| SpecializedSampleTypes | FFPE,LowInput |
| Technology | Sequencing |
| AutomationCapABIlity | LiquidHandlingRobots |
| VariantClass | NovelTranscripts,TranscriptVariants |
| Method | miRNAandSmallRNASequencing |
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我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!

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