
ProductHighlights:
TruSeqSmallRNALibraryPreparationKitsprovidereagentstogeneratesmallRNAlibrariesdirectlyfromtotalRNA.MicroRNAs(miRNAs)generatedbyDicerprocessingareefficientlytargetedbytheincludedmodifiedadapters.
Thesekitsenablemultiplexedsequencingwiththeintroductionof48uniqueindexes,allowingmiRNAandsmallRNAdiscoveryandprofilingthroughputtomatchtheunparalleledoutputofIlluminasequencing.Indexesareaddedinauniversalamplificationreaction,greatlyreducingligationbiasandensuringaccuratemeasurementofmiRNAexpression.
Workflowimprovementsenablestreamlinedsamplepreparation,allowingeconomicalstudiescoveringallsmallRNAtranscriptsinanyspecies.CompatIBLeapplicationsincludefindingnovelmiRNAs,characterizingvariationsuchasisomerswithsingle-baseresolution,andanalyzingdifferentialexpressionwithoutpriorassumptions.
TruSeqSmallRNASampleDatasets
TheHumanBrainReferenceRNA(HBRR)andUniversalHumanReferenceRNA(UHRR)sampleswerepreparedusingtheTruSeqSmallRNALibraryPrepKit.TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x36bpreadlengthconfiguration.Thetotalyieldwas0.91Gbwith97%ofbasesatoraboveQ30.
BrowsetheDataInBaseSpaceSequenceHub:
- ViewRun(primaryanalysisandmetrics)
- ViewProject(secondaryanalysiswithBaseSpaceApps)
Specifications:
AssayTime | ~10.5hours |
Hands-OnTime | ~30minutes |
InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
MechanismofAction | Oligo-dTbeadscapturepolyAtails |
Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
SampleThroughput | Upto16samplesperNeoPreplibrarycard |
SpeciesCategory | Drosophila,AnySpecies,Mouse,Zebrafish,Human,Rat,Plant,Nematode |
SystemCompatibility | MiSeq,NextSeq550,MiniSeq,NextSeq500 |
SpecializedSampleTypes | FFPE,LowInput |
Technology | Sequencing |
AutomationCapABIlity | LiquidHandlingRobots |
VariantClass | NovelTranscripts,TranscriptVariants |
Method | miRNAandSmallRNASequencing |
ebiomall.com






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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!


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