ProductHighlights:
TruSeqSmallRNALibraryPreparationKitsprovidereagentstogeneratesmallRNAlibrariesdirectlyfromtotalRNA.MicroRNAs(miRNAs)generatedbyDicerprocessingareefficientlytargetedbytheincludedmodifiedadapters.
Thesekitsenablemultiplexedsequencingwiththeintroductionof48uniqueindexes,allowingmiRNAandsmallRNAdiscoveryandprofilingthroughputtomatchtheunparalleledoutputofIlluminasequencing.Indexesareaddedinauniversalamplificationreaction,greatlyreducingligationbiasandensuringaccuratemeasurementofmiRNAexpression.
Workflowimprovementsenablestreamlinedsamplepreparation,allowingeconomicalstudiescoveringallsmallRNAtranscriptsinanyspecies.CompatIBLeapplicationsincludefindingnovelmiRNAs,characterizingvariationsuchasisomerswithsingle-baseresolution,andanalyzingdifferentialexpressionwithoutpriorassumptions.
TruSeqSmallRNASampleDatasets
TheHumanBrainReferenceRNA(HBRR)andUniversalHumanReferenceRNA(UHRR)sampleswerepreparedusingtheTruSeqSmallRNALibraryPrepKit.TheselibrariesweresequencedontheMiniSeqSystemusingtheMiniSeqHighOutputReagentKitata1x36bpreadlengthconfiguration.Thetotalyieldwas0.91Gbwith97%ofbasesatoraboveQ30.
BrowsetheDataInBaseSpaceSequenceHub:
- ViewRun(primaryanalysisandmetrics)
- ViewProject(secondaryanalysiswithBaseSpaceApps)
Specifications:
| AssayTime | ~10.5hours |
| Hands-OnTime | ~30minutes |
| InputQuantity | 25to100ngtotalRNAfromspecieswithpolyAtails |
| ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
| MechanismofAction | Oligo-dTbeadscapturepolyAtails |
| Multiplexing | Upto24-plexpoolingwithadditionalNeoPrepruns |
| SampleThroughput | Upto16samplesperNeoPreplibrarycard |
| SpeciesCategory | Drosophila,AnySpecies,Mouse,Zebrafish,Human,Rat,Plant,Nematode |
| SystemCompatibility | MiSeq,NextSeq550,MiniSeq,NextSeq500 |
| SpecializedSampleTypes | FFPE,LowInput |
| Technology | Sequencing |
| AutomationCapABIlity | LiquidHandlingRobots |
| VariantClass | NovelTranscripts,TranscriptVariants |
| Method | miRNAandSmallRNASequencing |
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?

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