

Description: TheMCNext™SYBR®FastqPCRLibraryQuantificationKitprovidesafastandreliablesolutiontodeterminethelibraryconcentrationwithadirectestimateclusterdensity.ThisdirectmeasurementmethodsignificantlysavestimeandresourcescomparingwithotherqPCRlibraryquantificationkitsonthemarket.ThisisachievedbyprovidingaconstructedlibraryratherthanasingleDNAfragmentasacontrolstandardwithknownclustergenerationdensityonIllumina®sequencingplatforms. TheMCNext™SYBR®FastqPCRLibraryQuantificationKitutilizesMCLAB’shighperformancefastPCRenzymebyaPhiXgenomebasedLibraryStandards(forseven10-folddilutions)withanaveragesizeof570bp,providingquickandaccuratequantificationwith0.0001–100pMdynamicrangein60min.Basedonthequantification,theresultcouldbedirectlyconvertedtoclusternumbersforloadingvolumereferences. TheMCNext™SYBR®FastqPCRLibraryQuantificationKitisarapidsolutionforyourlibraryconstructionQCtoaddvaluetoyourworkflowandincreaseconfidenceinyourresults.TheproprietaryPhiXLibraryStandardsconsistofagroupofIllumina®sequencing adapter-ligatedDNAfragmentsderivedfromthePhiXgenomewithwell-characterizedsequenceandwell-balancedGCcontent(50%).TousethislibraryasacontrolforlibrariesconstructedforsequencingonIllumina®sequencingplatformsisfarsuperiorthanasingleDNAfragmentascontrolstandardusedbyotherqPCRquantificationkitsonthecurrentmarket.Itcanberapidlyinterpolatedfortheestimatingclusterdensityandservesasaquantificationbaselineforsamplelibrarieswithouttheneedtohaveanothersequencingrunforaclusterdensitymeasurementpurpose.Itisanexcellentcontrolwithmeasuredclustergenerationconversionparametertoexaminethelibraryqualityandquantity,allowsyoutoquicklydetermineifanerrorisrelatedtothesamplepreparationbeforeahigh-costsequencingrun.Quantificationisfast,directandaccuratebyextrapolationagainstthestandardcurvegeneratedusingtheseven10-foldLibraryStandardsdilutions,followedbyasimpleclusternumberconversion. TheMCNext™SYBR®FastqPCRLibraryQuantificationKitisavailablein3types(RegularROX,LowROXandUniversal)basedontheinternalreferencedyepreferencesofthePCRThermocyclers.Thepackagesincludeready-to-use2XMasterMix,10XPrimerMixandPhiXLibraryStandards. Features TheMCNext™SYBR®FastqPCRLibraryQuantificationKitprovidesresearcherswithanaccurateandsensitivemethodforquantifyingNGSlibraries.
ConvenientpackagesforalltypesofrealtimePCRplatforms |
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我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!

