AntibodyDilution | WB(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser. |
Conjugates | AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated
PerCP | Overview:- Peridinin-Chlorophyll-ProteinComplex
- Smallphycobiliprotein
- Isolatedfromredalgae
- Largestokesshift(195nm)
- MolecularWeight:35kDa
PerCPDatasheet |  | OpticalProperties: λex =482nm λem=677nm εmax=1.96x106 Laser =488nm PE/ATTO594 | PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm. | Overview:- Highfluorescenceyield
- HighphotostABIlity
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
PE/ATTO594Datasheet |  | OpticalProperties: λex =535nm λem =627 nm Laser = 488to561nm FITC(Fluorescein) | Overview:- Excellentfluorescencequantumyield
- Highrateofphotobleaching
- Goodsolubilityinwater
- Broademissionspectrum
- pHdependentspectra
- Molecularformula:C20H12O5
- Molarmass:332.3g/mol
FITC-Fluorescent-conjugate |  | OpticalProperties: λex =494 nm λem=520 nm εmax=7.3×104 Φf= 0.92 τfl =5.0ns Brightness= 67.2 Laser = 488 nm Filterset = FITC ATTO700 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:575g/mol
ATTO700Datasheet |  | OpticalProperties: λex =700 nm λem=719nm εmax=1.25×105 Φf= 0.25 τfl =1.6ns Brightness= 31.3 Laser =676nm Filterset =Cy®5.5 ATTO680 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:631g/mol
ATTO680Datasheet |  | OpticalProperties: λex =680nm λem=700 nm εmax=1.25×105 Φf= 0.30 τfl =1.7ns Brightness=37.5 Laser =633 –676nm Filterset =Cy®5.5 ATTO655 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Excellentozoneresistance
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:634g/mol
ATTO655Datasheet |  | OpticalProperties: λex =663nm λem=684nm εmax=1.25×105 Φf= 0.30 τfl =1.8ns Brightness= 37.5 Laser =633 –647nm Filterset =Cy®5 ATTO633 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- StableatpH4–11
- Cationicdye,perchloratesalt
- MolarMass:652.2g/mol
ATTO633Datasheet |  | OpticalProperties: λex =629nm λem=657nm εmax=1.3×105 Φf= 0.64 τfl =3.2ns Brightness=83.2 Laser =633 nm Filterset =Cy®5 ATTO594 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:1137g/mol
ATTO594Datasheet |  | OpticalProperties: λex =601 nm λem=627nm εmax=1.2×105 Φf= 0.85 τfl =3.5ns Brightness=102 Laser =594nm Filterset =TexasRed® ATTO565 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Goodsolubilityinpolarsolvents
- Excellentsolubilityinwater
- Verylittleaggregation
- Rhodaminedyederivative
- MolarMass:611g/mol
ATTO565Datasheet |  | OpticalProperties: λex =563nm λem=592nm εmax=1.2×105 Φf= 0.9 τfl =3.4n Brightness=10 Laser =532nm Filterset = TRITC ATTO488 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:804g/mol
ATTO488Datasheet |  | OpticalProperties: λex =501nm λem=523nm εmax=9.0×104 Φf= 0.80 τfl =4.1ns Brightness= 72 Laser = 488 nm Filterset = FITC ATTO390 | Overview:- Highfluorescenceyield
- LargeStokes-shift(89nm)
- Goodphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- Coumarinderivate,uncharged
- Lowmolarmass:343.42g/mol
ATTO390Datasheet |  | OpticalProperties: λex =390nm λem=479nm εmax=2.4×104 Φf= 0.90 τfl =5.0ns Brightness= 21.6 Laser =365or405nm APC(Allophycocyanin) | Overview:- Highquantumyield
- Largephycobiliprotein
- 6chromophorespermolecule
- Isolatedfromredalgae
- MolecularWeight:105kDa
APCDatasheet |  | OpticalProperties: λex =650nm λem=660nm εmax=7.0×105 Φf= 0.68 Brightness= 476 Laser =594or633 nm Filterset =Cy®5 StreptavidinProperties: - Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
- Molecularweight:53kDa
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
StreptavidinDatasheet Biotin Properties: - BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
- Molarmass:244.31g/mol
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
BiotinDatasheet HRP(HorserADIshperoxidase)Properties: - Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
- Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
- Highturnoverrateenablesrapidgenerationofastrongsignal
- 44kDaglycoprotein
- Extinctioncoefficient:100(403nm)
- Applications:Westernblot,immunohistochemistry,andELISA
HRPDatasheet AP(AlkalinePhosphatase)Properties: - Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
- Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
- Molecularweight:140kDa
- Applications:Westernblot,immunohistochemistry,andELISA
APDatasheet R-PE(R-Phycoerythrin) | Overview:- Broadexcitationspectrum
- Highquantumyield
- Photostable
- Memberofthephycobiliproteinfamily
- Isolatedfromredalgae
- Excellentsolubilityinwater
- MolecularWeight:250kDa
R-PEDatasheet |  | OpticalProperties: λex =565nm λem=575nm εmax=2.0×106 Φf= 0.84 Brightness=1.68x103 Laser = 488to561nm Filterset = TRITC ProductImages Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-DNMT1MonoclonalAntibody,Clone11H8(SMC-231).Tissue:Coloncarcinomacellline(RKO).Species:Human.Fixation:4%Formaldehydefor15minatRT.PrimaryAntibody:MouseAnti-DNMT1MonoclonalAntibody(SMC-231)at1:100for60minatRT.SecondaryAntibody:GoatAnti-MouseATTO488at1:100for60minatRT.Counterstain:PhalloidinTexasRedF-Actinstain;DAPI(blue)nuclearstainat1:1000,1:5000for60minatRT,5minatRT.Localization:Nucleus.Magnification:60X.(A)DAPInuclearstain.(B)PhalloidinTexasRedF-Actinstain.(C)DNMT1.(D)Composite. ProductCitations(0)Currentlytherearenocitationsforthisproduct. FITC(Fluorescein) | Overview:- Excellentfluorescencequantumyield
- Highrateofphotobleaching
- Goodsolubilityinwater
- Broademissionspectrum
- pHdependentspectra
- Molecularformula:C20H12O5
- Molarmass:332.3g/mol
FITC-Fluorescent-conjugate |  | OpticalProperties: λex =494 nm λem=520 nm εmax=7.3×104 Φf= 0.92 τfl =5.0ns Brightness= 67.2 Laser = 488 nm Filterset = FITC
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B7703S-8 ml8 mlnebNew England BiolabsNEB/Blue Loading Buffer Pack/B7703S/8 ml 查看更多>
北京索宝生物科技有限公司在发布的荧光蛋白上样缓冲液供应信息,浏览与荧光蛋白上样缓冲液相关的产品或在搜索更多与荧光蛋白上样缓冲液相关的内容。 查看更多>
5×SDS-PAGE蛋白上样缓冲液(25甘油) 查看更多>
Description: 60% Glycerol, 0.25% bromophenol blue, 20 mL, Non-Sterile. 10 Pack. Application: For agarose gels, DNA or RNA Shipping Conditions:Room Temperature Storage Conditions: Room Temperature. 查看更多>
北京绿源伯德生物科技有限公司在发布的RNA LOADING BUFFER,2X RNA上样缓冲液,2X供应信息,浏览与RNA LOADING BUFFER,2X RNA上样缓冲液,2X相关的产品或在搜索更多与RNA LOADING BUFFER,2X RNA上样缓冲液,2X相关的内容。 查看更多>
No prestaining "Prestaining" with GRGreen 查看更多>
北京索宝生物科技有限公司在发布的4×蛋白上样缓冲液(含DTT)供应信息,浏览与4×蛋白上样缓冲液(含DTT)相关的产品或在搜索更多与4×蛋白上样缓冲液(含DTT)相关的内容。 查看更多>
ABIN11130355 mLantibodies-onlineAntibodies-Online,inc.Antibodies-Online/Native Gel Sample Loading Buffer (5x)/ABIN1113035/5 mL 查看更多>
本页关键字:amresco蛋白电泳上样缓冲液 amresco报价 amresco蛋白电泳上样缓冲液,amresco报价。提供amresco蛋白电泳上样缓冲液报价、咨询及技术服务,欢迎来电订购amresco蛋白电泳上样缓冲液。中国试剂网经营实验试剂、实验耗材、实验仪器及实验技术服务。公司简介 Amresco 是全球著名的生化试剂生产商,其产品种类齐全,质量稳定,性价比好。深受广大用户的喜爱。Amresco公司来自美国,成立于 1976 年 查看更多>
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RNA Loading Buffer (+EB) 500 μl × 2支
□ RNA Loading Buffer组成
Formamide 62.5%
Formaldehyde 0.4 M
20 × MOPS Buffer 1.25×
Xylene Cyanol FF 0.02%
Bromophenol Blue 0.02%
Ethidium Bromide 0.0025% 查看更多>
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实验室的一份protocol 上写配上样缓冲液的时候要加溴酚蓝bromophenol blue,但没写加的量是多少。实验室只有粉末状的溴酚蓝。请问大神们如何加,加多少啊?需要先把溴酚蓝配制成溶液吗?还是直接加进去?
上样缓冲液也有多种,一般是80ul的样本加20ul的上样缓冲液,这样基本上上样50ug的体积在10ul左右。 最好是测浓度,一般不超过30-40ug,上样缓冲液一般4×或者5×,这个没太大区别。
实验小白刚刚起步,一般采用等质量上样的方法,但我算的上样量是没加缓冲液之前的,加完缓冲液蛋白样品浓度会变么?师姐说不会变,就按照算好的上样量加就行。 前几天提的样按50ug算每个样本的上样量也只有上5ul左右,按师姐说的就那么上了,结果发完光只有内参有条带,目标蛋白一点也没有,但以前做过一次是出过一点趋势的,而且这次整个过程感觉做的很仔细,发完光结果也很干净整齐,所以我在想是不是跟上样量有关系?上样缓冲液加入之后会使样本浓度稀释五倍么,这样的话上样量就得乘5。 变性前后加上样缓冲液的区别是什么呢?哪一种会改变蛋白浓度从而改变上样量呢?
蛋白上样缓冲液 蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致,减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态,保证蛋白分子的线性. 溴酚蓝作用是指示上样蛋白的跑胶时标记的,甘油是增加上样重量的,以免漂浮,煮沸是使蛋白变性的永久保存
提了蛋白,加上样缓冲液煮过,但发现还是有很多胶状的沉淀,比较黏稠,堵枪头。于是自作主张又超声了一次,结果跑了一次,内参都没有。煮过之后的蛋白不能超声么?
2ul 5×上样缓冲液中添加3ul水,混匀,即得2×上样缓冲液
电泳缓冲液的主要作用是使胶的导电性和体系的一致,这样跑出的条带才回一致; 加样缓冲液的主要作用是使PCR产物与其混合, 使DNA沉于加样孔的底部,防止DNA跑出来.
(以下内容仅供参考) 2乘SDS-PAGE上样缓冲液(20ml体系) 1.0mol/L Tris-HCL (pH 6.8) 2ml 1.0mol/L DTT 4ml SDS 0.8g 溴芬蓝 0.04g 甘油 4ml dd水 定容至20ml 4℃冰箱保存,可以不分装,我们实验室一般用三个月以上 先配1.0mol/L的DTT,双蒸无菌水溶解4℃冰箱保存,配上样缓冲液的时候加入就可以了,都混匀保存也没问题的
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